TY - JOUR
T1 - Evaluation of the SD bioline cholera rapid diagnostic test during the 2016 cholera outbreak in Lusaka, Zambia
AU - Mwaba, John
AU - Ferreras, Eva
AU - Chizema-Kawesa, Elizabeth
AU - Mwimbe, Daniel
AU - Tafirenyika, Francis
AU - Rauzier, Jean
AU - Blake, Alexandre
AU - Rakesh, Ankur
AU - Poncin, Marc
AU - Stoitsova, Savina
AU - Kwenda, Geoffrey
AU - Azman, Andrew S.
AU - Chewe, Orbrie
AU - Serafini, Micaela
AU - Lukwesa-Musyani, Chileshe
AU - Cohuet, Sandra
AU - Quilici, Marie Laure
AU - Luquero, Francisco J.
AU - Page, Anne Laure
N1 - Funding Information:
We are grateful to all study participants. We wish to thank UTH microbiology members of staff who were very helpful to the team during running of PCR. We thank Children Kaluha and Iza Ciglenecki for their support to the study and Dr Roma Chilengi for critical reading of the manuscript. This work was supported by Médecins Sans Frontières – Operational Center Geneva.
Publisher Copyright:
© 2018 John Wiley & Sons Ltd.
PY - 2018
Y1 - 2018
N2 - objective To assess the performance of the SD Bioline Cholera Ag O1/O139 rapid diagnostic test (RDT) compared to a reference standard combining culture and PCR for the diagnosis of cholera cases during an outbreak. methods RDT and bacterial culture were performed on site using fresh stools collected from cholera suspected cases, and from stools enriched in alkaline peptone water. Dried stool samples on filter paper were tested for V. cholerae by PCR in Lusaka (as part of a laboratory technology transfer project) and at a reference laboratory in Paris, France. A sample was considered positive for cholera by the reference standard if any of the culture or PCR tests was positive for V. cholerae O1 or O139. results Among the 170 samples tested with SD Bioline and compared to the reference standard, the RDT showed a sensitivity of 90.9%(95%CI: 81.3–96.6) and specificity of 95.2% (95%CI: 89.1–98.4). After enrichment, the sensitivity was 95.5% (95%CI: 87.3–99.1) and specificity 100%(95%CI: 96.5–100). conclusion The observed sensitivity and specificity were within recommendations set by the Global Task Force for Cholera Control on the use of cholera RDT (sensitivity = 90%; specificity = 85%). Although the sample size was small, our findings suggest that the SD Bioline RDT could be used in the field to rapidly alert public health officials to the likely presence of cholera cases when an outbreak is suspected.
AB - objective To assess the performance of the SD Bioline Cholera Ag O1/O139 rapid diagnostic test (RDT) compared to a reference standard combining culture and PCR for the diagnosis of cholera cases during an outbreak. methods RDT and bacterial culture were performed on site using fresh stools collected from cholera suspected cases, and from stools enriched in alkaline peptone water. Dried stool samples on filter paper were tested for V. cholerae by PCR in Lusaka (as part of a laboratory technology transfer project) and at a reference laboratory in Paris, France. A sample was considered positive for cholera by the reference standard if any of the culture or PCR tests was positive for V. cholerae O1 or O139. results Among the 170 samples tested with SD Bioline and compared to the reference standard, the RDT showed a sensitivity of 90.9%(95%CI: 81.3–96.6) and specificity of 95.2% (95%CI: 89.1–98.4). After enrichment, the sensitivity was 95.5% (95%CI: 87.3–99.1) and specificity 100%(95%CI: 96.5–100). conclusion The observed sensitivity and specificity were within recommendations set by the Global Task Force for Cholera Control on the use of cholera RDT (sensitivity = 90%; specificity = 85%). Although the sample size was small, our findings suggest that the SD Bioline RDT could be used in the field to rapidly alert public health officials to the likely presence of cholera cases when an outbreak is suspected.
KW - Cholera
KW - Diagnostic accuracy
KW - Outbreak
KW - Rapid diagnostic test
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U2 - 10.1111/tmi.13084
DO - 10.1111/tmi.13084
M3 - Article
C2 - 29851181
AN - SCOPUS:85054759322
SN - 1360-2276
VL - 23
SP - 834
EP - 840
JO - Tropical Medicine and International Health
JF - Tropical Medicine and International Health
IS - 8
ER -