Evaluation of the SD bioline cholera rapid diagnostic test during the 2016 cholera outbreak in Lusaka, Zambia

John Mwaba, Eva Ferreras, Elizabeth Chizema-Kawesa, Daniel Mwimbe, Francis Tafirenyika, Jean Rauzier, Alexandre Blake, Ankur Rakesh, Marc Poncin, Savina Stoitsova, Geoffrey Kwenda, Andrew Azman, Orbrie Chewe, Micaela Serafini, Chileshe Lukwesa-Musyani, Sandra Cohuet, Marie Laure Quilici, Francisco J. Luquero, Anne Laure Page

Research output: Contribution to journalArticle

Abstract

objective To assess the performance of the SD Bioline Cholera Ag O1/O139 rapid diagnostic test (RDT) compared to a reference standard combining culture and PCR for the diagnosis of cholera cases during an outbreak. methods RDT and bacterial culture were performed on site using fresh stools collected from cholera suspected cases, and from stools enriched in alkaline peptone water. Dried stool samples on filter paper were tested for V. cholerae by PCR in Lusaka (as part of a laboratory technology transfer project) and at a reference laboratory in Paris, France. A sample was considered positive for cholera by the reference standard if any of the culture or PCR tests was positive for V. cholerae O1 or O139. results Among the 170 samples tested with SD Bioline and compared to the reference standard, the RDT showed a sensitivity of 90.9%(95%CI: 81.3–96.6) and specificity of 95.2% (95%CI: 89.1–98.4). After enrichment, the sensitivity was 95.5% (95%CI: 87.3–99.1) and specificity 100%(95%CI: 96.5–100). conclusion The observed sensitivity and specificity were within recommendations set by the Global Task Force for Cholera Control on the use of cholera RDT (sensitivity = 90%; specificity = 85%). Although the sample size was small, our findings suggest that the SD Bioline RDT could be used in the field to rapidly alert public health officials to the likely presence of cholera cases when an outbreak is suspected.

Original languageEnglish (US)
Pages (from-to)834-840
Number of pages7
JournalTropical Medicine and International Health
Volume23
Issue number8
DOIs
StatePublished - Jan 1 2018

Fingerprint

Zambia
Cholera
Routine Diagnostic Tests
Disease Outbreaks
Polymerase Chain Reaction
Technology Transfer
Peptones
Paris
Advisory Committees
Sample Size
France
Public Health
Sensitivity and Specificity

Keywords

  • Cholera
  • Diagnostic accuracy
  • Outbreak
  • Rapid diagnostic test

ASJC Scopus subject areas

  • Parasitology
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Mwaba, J., Ferreras, E., Chizema-Kawesa, E., Mwimbe, D., Tafirenyika, F., Rauzier, J., ... Page, A. L. (2018). Evaluation of the SD bioline cholera rapid diagnostic test during the 2016 cholera outbreak in Lusaka, Zambia. Tropical Medicine and International Health, 23(8), 834-840. https://doi.org/10.1111/tmi.13084

Evaluation of the SD bioline cholera rapid diagnostic test during the 2016 cholera outbreak in Lusaka, Zambia. / Mwaba, John; Ferreras, Eva; Chizema-Kawesa, Elizabeth; Mwimbe, Daniel; Tafirenyika, Francis; Rauzier, Jean; Blake, Alexandre; Rakesh, Ankur; Poncin, Marc; Stoitsova, Savina; Kwenda, Geoffrey; Azman, Andrew; Chewe, Orbrie; Serafini, Micaela; Lukwesa-Musyani, Chileshe; Cohuet, Sandra; Quilici, Marie Laure; Luquero, Francisco J.; Page, Anne Laure.

In: Tropical Medicine and International Health, Vol. 23, No. 8, 01.01.2018, p. 834-840.

Research output: Contribution to journalArticle

Mwaba, J, Ferreras, E, Chizema-Kawesa, E, Mwimbe, D, Tafirenyika, F, Rauzier, J, Blake, A, Rakesh, A, Poncin, M, Stoitsova, S, Kwenda, G, Azman, A, Chewe, O, Serafini, M, Lukwesa-Musyani, C, Cohuet, S, Quilici, ML, Luquero, FJ & Page, AL 2018, 'Evaluation of the SD bioline cholera rapid diagnostic test during the 2016 cholera outbreak in Lusaka, Zambia', Tropical Medicine and International Health, vol. 23, no. 8, pp. 834-840. https://doi.org/10.1111/tmi.13084
Mwaba, John ; Ferreras, Eva ; Chizema-Kawesa, Elizabeth ; Mwimbe, Daniel ; Tafirenyika, Francis ; Rauzier, Jean ; Blake, Alexandre ; Rakesh, Ankur ; Poncin, Marc ; Stoitsova, Savina ; Kwenda, Geoffrey ; Azman, Andrew ; Chewe, Orbrie ; Serafini, Micaela ; Lukwesa-Musyani, Chileshe ; Cohuet, Sandra ; Quilici, Marie Laure ; Luquero, Francisco J. ; Page, Anne Laure. / Evaluation of the SD bioline cholera rapid diagnostic test during the 2016 cholera outbreak in Lusaka, Zambia. In: Tropical Medicine and International Health. 2018 ; Vol. 23, No. 8. pp. 834-840.
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T1 - Evaluation of the SD bioline cholera rapid diagnostic test during the 2016 cholera outbreak in Lusaka, Zambia

AU - Mwaba, John

AU - Ferreras, Eva

AU - Chizema-Kawesa, Elizabeth

AU - Mwimbe, Daniel

AU - Tafirenyika, Francis

AU - Rauzier, Jean

AU - Blake, Alexandre

AU - Rakesh, Ankur

AU - Poncin, Marc

AU - Stoitsova, Savina

AU - Kwenda, Geoffrey

AU - Azman, Andrew

AU - Chewe, Orbrie

AU - Serafini, Micaela

AU - Lukwesa-Musyani, Chileshe

AU - Cohuet, Sandra

AU - Quilici, Marie Laure

AU - Luquero, Francisco J.

AU - Page, Anne Laure

PY - 2018/1/1

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N2 - objective To assess the performance of the SD Bioline Cholera Ag O1/O139 rapid diagnostic test (RDT) compared to a reference standard combining culture and PCR for the diagnosis of cholera cases during an outbreak. methods RDT and bacterial culture were performed on site using fresh stools collected from cholera suspected cases, and from stools enriched in alkaline peptone water. Dried stool samples on filter paper were tested for V. cholerae by PCR in Lusaka (as part of a laboratory technology transfer project) and at a reference laboratory in Paris, France. A sample was considered positive for cholera by the reference standard if any of the culture or PCR tests was positive for V. cholerae O1 or O139. results Among the 170 samples tested with SD Bioline and compared to the reference standard, the RDT showed a sensitivity of 90.9%(95%CI: 81.3–96.6) and specificity of 95.2% (95%CI: 89.1–98.4). After enrichment, the sensitivity was 95.5% (95%CI: 87.3–99.1) and specificity 100%(95%CI: 96.5–100). conclusion The observed sensitivity and specificity were within recommendations set by the Global Task Force for Cholera Control on the use of cholera RDT (sensitivity = 90%; specificity = 85%). Although the sample size was small, our findings suggest that the SD Bioline RDT could be used in the field to rapidly alert public health officials to the likely presence of cholera cases when an outbreak is suspected.

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KW - Diagnostic accuracy

KW - Outbreak

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