Evaluation of the Roche AMPLICOR enterovirus PCR assay in the diagnosis of enteroviral central nervous system infections

Karen C Carroll, Bill Taggart, John Robison, Carrie Byington, David Hillyard

Research output: Contribution to journalArticle

Abstract

Background: Enteroviruses cause a substantial number of cases of aseptic meningitis annually in the USA. While culture has been useful in the detection of patients with viral meningitis it is time-consuming and lacks sensitivity. Detection of viral nucleic acid in patient specimens has been demonstrated to improve enteroviral detection. Objectives: A research use only commercial amplification assay, the Roche AMPLICOR EV test, was compared to culture for the diagnosis of enteroviral meningoencephalitis. Study Design: Four-hundred and sixty-five consecutive CSF samples sent prospectively for suspicion of enteroviral infection were evaluated by PCR and shell-vial culture. Clinical information and CSF analysis were used to resolve PCR positive, culture negative samples. Sensitivity and specificity were calculated using resolved data. Results: There were 138 samples which met the definition of a true positive. Of these culture detected 77 (sensitivity 55.8%) and PCR detected 136 (sensitivity 98.6%). PCR missed two culture positive samples. Upon repeat testing, these CSF samples were found to contain inhibitors. Conclusions: The Roche AMPLICOR EV-PCR test was statistically more sensitive than culture (P

Original languageEnglish (US)
Pages (from-to)149-156
Number of pages8
JournalJournal of Clinical Virology
Volume19
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Central Nervous System Infections
Enterovirus
Polymerase Chain Reaction
Viral Meningitis
Aseptic Meningitis
Meningoencephalitis
Nucleic Acids
Sensitivity and Specificity
Infection
Research

Keywords

  • Enteroviruses
  • Meningoencephalitis
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Evaluation of the Roche AMPLICOR enterovirus PCR assay in the diagnosis of enteroviral central nervous system infections. / Carroll, Karen C; Taggart, Bill; Robison, John; Byington, Carrie; Hillyard, David.

In: Journal of Clinical Virology, Vol. 19, No. 3, 2000, p. 149-156.

Research output: Contribution to journalArticle

Carroll, Karen C ; Taggart, Bill ; Robison, John ; Byington, Carrie ; Hillyard, David. / Evaluation of the Roche AMPLICOR enterovirus PCR assay in the diagnosis of enteroviral central nervous system infections. In: Journal of Clinical Virology. 2000 ; Vol. 19, No. 3. pp. 149-156.
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