Evaluation of the prototype Roche DNA amplification kit incorporating the new SSK145 and SKCC1B primers in detection of human immunodeficiency virus type 1 DNA in Zimbabwe

Lynn S. Zijenah, Jean Hawes Humphrey, Kussum Nathoo, Lucie Malaba, Parteson Zvandasara, Agnes Mahomva, Peter Iliff, Michael T. Mbizvo

Research output: Contribution to journalArticle

Abstract

We assessed the sensitivity and specificity of a newly developed DNA PCR kit (Roche Diagnostic Corporation, Indianapolis, Ind.) that incorporates primers for all the group M viruses for the detection of human immunodeficiency virus (HIV) type 1 (HIV - 1) infection in Zimbabwe. A total of 202 whole-blood samples from adults whose HIV status was known were studied. This included 100 HIV-1-positive and 102 HIV-1-negative samples selected on the basis of concordant results obtained with two enzyme-linked immunosorbent assay kits. The prototype Roche DNA PCR assay had a 100% sensitivity for the detection of HIV-1 DNA and a specificity of 100%. We conclude that the new Roche DNA PCR kit is accurate for the detection of HIV DNA in Zimbabwean samples, in which HIV-1 subtype C dominates.

Original languageEnglish (US)
Pages (from-to)3569-3571
Number of pages3
JournalJournal of Clinical Microbiology
Volume37
Issue number11
StatePublished - Nov 1999
Externally publishedYes

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Zimbabwe
HIV-1
DNA
Polymerase Chain Reaction
HIV
Virus Diseases
Enzyme-Linked Immunosorbent Assay
Viruses
Sensitivity and Specificity

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Evaluation of the prototype Roche DNA amplification kit incorporating the new SSK145 and SKCC1B primers in detection of human immunodeficiency virus type 1 DNA in Zimbabwe. / Zijenah, Lynn S.; Humphrey, Jean Hawes; Nathoo, Kussum; Malaba, Lucie; Zvandasara, Parteson; Mahomva, Agnes; Iliff, Peter; Mbizvo, Michael T.

In: Journal of Clinical Microbiology, Vol. 37, No. 11, 11.1999, p. 3569-3571.

Research output: Contribution to journalArticle

Zijenah, Lynn S. ; Humphrey, Jean Hawes ; Nathoo, Kussum ; Malaba, Lucie ; Zvandasara, Parteson ; Mahomva, Agnes ; Iliff, Peter ; Mbizvo, Michael T. / Evaluation of the prototype Roche DNA amplification kit incorporating the new SSK145 and SKCC1B primers in detection of human immunodeficiency virus type 1 DNA in Zimbabwe. In: Journal of Clinical Microbiology. 1999 ; Vol. 37, No. 11. pp. 3569-3571.
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