TY - JOUR
T1 - Evaluation of the prototype Roche DNA amplification kit incorporating the new SSK145 and SKCC1B primers in detection of human immunodeficiency virus type 1 DNA in Zimbabwe
AU - Zijenah, Lynn S.
AU - Humphrey, Jean
AU - Nathoo, Kussum
AU - Malaba, Lucie
AU - Zvandasara, Parteson
AU - Mahomva, Agnes
AU - Iliff, Peter
AU - Mbizvo, Michael T.
PY - 1999/11
Y1 - 1999/11
N2 - We assessed the sensitivity and specificity of a newly developed DNA PCR kit (Roche Diagnostic Corporation, Indianapolis, Ind.) that incorporates primers for all the group M viruses for the detection of human immunodeficiency virus (HIV) type 1 (HIV - 1) infection in Zimbabwe. A total of 202 whole-blood samples from adults whose HIV status was known were studied. This included 100 HIV-1-positive and 102 HIV-1-negative samples selected on the basis of concordant results obtained with two enzyme-linked immunosorbent assay kits. The prototype Roche DNA PCR assay had a 100% sensitivity for the detection of HIV-1 DNA and a specificity of 100%. We conclude that the new Roche DNA PCR kit is accurate for the detection of HIV DNA in Zimbabwean samples, in which HIV-1 subtype C dominates.
AB - We assessed the sensitivity and specificity of a newly developed DNA PCR kit (Roche Diagnostic Corporation, Indianapolis, Ind.) that incorporates primers for all the group M viruses for the detection of human immunodeficiency virus (HIV) type 1 (HIV - 1) infection in Zimbabwe. A total of 202 whole-blood samples from adults whose HIV status was known were studied. This included 100 HIV-1-positive and 102 HIV-1-negative samples selected on the basis of concordant results obtained with two enzyme-linked immunosorbent assay kits. The prototype Roche DNA PCR assay had a 100% sensitivity for the detection of HIV-1 DNA and a specificity of 100%. We conclude that the new Roche DNA PCR kit is accurate for the detection of HIV DNA in Zimbabwean samples, in which HIV-1 subtype C dominates.
UR - http://www.scopus.com/inward/record.url?scp=0032862034&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032862034&partnerID=8YFLogxK
U2 - 10.1128/jcm.37.11.3569-3571.1999
DO - 10.1128/jcm.37.11.3569-3571.1999
M3 - Article
C2 - 10523553
AN - SCOPUS:0032862034
SN - 0095-1137
VL - 37
SP - 3569
EP - 3571
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 11
ER -