Evaluation of the Prodesse Hexaplex multiplex PCR assay for direct detection of seven respiratory viruses in clinical specimens

M. Hindiyeh, D. R. Hillyard, K. C. Carroll

Research output: Contribution to journalArticle

Abstract

We evaluated the Hexaplex assay (Prodesse, Waukesha, WI) for the detection of 7 respiratory viruses (influenza A and B, parainfluenza 1-3, and respiratory syncytial virus [RSV] A and B). The Hexaplex assay was performed on 300 respiratory samples during the 1999-2000 respiratory virus season. Results of this assay were compared with shell vial cell culture and/or direct fluorescent antibody stain. The overall sensitivity and specificity of the assay were 96.6% and 94.1%, respectively. The respective sensitivity and specificity of the Hexaplex assay for detection of specific virus groups were as follows: influenza A, 98.6% and 97.8%; influenza B, 100% and 100%; and for parainfluenza viruses (1-3), 100% and 99.1%. The assay did not perform as well with patients infected with RSV: sensitivity and specificity were 91.0% and 98.6%, respectively. There are 2 major drawbacks to this assay: it is technically demanding (3-4 hours hands-on time), and it is expensive ($80-$90 direct cost). Nevertheless, because of the excellent sensitivity and specificity; the Hexaplex assay may be valuable in the diagnosis of respiratory viral infections in immunocompromised patients.

Original languageEnglish (US)
Pages (from-to)218-224
Number of pages7
JournalAmerican journal of clinical pathology
Volume116
Issue number2
DOIs
StatePublished - Nov 20 2001
Externally publishedYes

Keywords

  • Human parainfluenza viruses
  • Influenza
  • Multiplex PCR
  • Respiratory syncytial viruses
  • Respiratory viruses

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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