Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets

Joong Hyun Kim; Seok Yeong Ko; Justin Ho Lee; Deok Ho Kim; Jeong Ho Yun

doi:10.5051/jpis.2017.47.6.402

Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets

Joong Hyun Kim, Seok Yeong Ko, Justin Ho Lee, Deok Ho Kim, Jeong Ho Yun

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Purpose: The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum. Methods: In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet. Results: The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets. Conclusions: This experiment confirmed that patterned cell sheets provide flexibility in designing hPDLSC sheets, and that these stem cell sheets may be candidates for application in periodontal regenerative therapy.

Original language	English (US)
Pages (from-to)	402-415
Number of pages	14
Journal	Journal of Periodontal and Implant Science
Volume	47
Issue number	6
DOIs	https://doi.org/10.5051/jpis.2017.47.6.402
State	Published - Dec 1 2017
Externally published	Yes

Keywords

Extracellular matrix
Periodontal ligament
Regeneration
Stem cells
Tissue engineering

ASJC Scopus subject areas

Oral Surgery
Periodontics

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10.5051/jpis.2017.47.6.402

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title = "Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets",

abstract = "Purpose: The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum. Methods: In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet. Results: The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets. Conclusions: This experiment confirmed that patterned cell sheets provide flexibility in designing hPDLSC sheets, and that these stem cell sheets may be candidates for application in periodontal regenerative therapy.",

keywords = "Extracellular matrix, Periodontal ligament, Regeneration, Stem cells, Tissue engineering",

author = "Kim, {Joong Hyun} and Ko, {Seok Yeong} and Lee, {Justin Ho} and Kim, {Deok Ho} and Yun, {Jeong Ho}",

note = "Funding Information: This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C0450). Publisher Copyright: {\textcopyright} 2017. Korean Academy of Periodontology.",

year = "2017",

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doi = "10.5051/jpis.2017.47.6.402",

language = "English (US)",

volume = "47",

pages = "402--415",

journal = "Journal of Periodontal and Implant Science",

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T1 - Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets

AU - Kim, Joong Hyun

AU - Ko, Seok Yeong

AU - Lee, Justin Ho

AU - Kim, Deok Ho

AU - Yun, Jeong Ho

N1 - Funding Information: This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C0450). Publisher Copyright: © 2017. Korean Academy of Periodontology.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Purpose: The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum. Methods: In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet. Results: The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets. Conclusions: This experiment confirmed that patterned cell sheets provide flexibility in designing hPDLSC sheets, and that these stem cell sheets may be candidates for application in periodontal regenerative therapy.

AB - Purpose: The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum. Methods: In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet. Results: The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets. Conclusions: This experiment confirmed that patterned cell sheets provide flexibility in designing hPDLSC sheets, and that these stem cell sheets may be candidates for application in periodontal regenerative therapy.

KW - Extracellular matrix

KW - Periodontal ligament

KW - Regeneration

KW - Stem cells

KW - Tissue engineering

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JO - Journal of Periodontal and Implant Science

JF - Journal of Periodontal and Implant Science

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Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets

Abstract

Keywords

ASJC Scopus subject areas

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