TY - JOUR
T1 - Evaluation of the Pan Influenza detection kit utilizing the PLEX-ID and influenza samples from the 2011 respiratory season
AU - Murillo, Luis A.
AU - Hardick, Justin
AU - Jeng, Kevin
AU - Gaydos, Charlotte A.
PY - 2013/10
Y1 - 2013/10
N2 - A comparison study was performed between the PLEX-ID and the CDC RT-PCR method for the detection and identification of Influenza A viruses using nasopharyngeal samples (N= 75) collected between January and May 2011. Overall agreement was 89.3% (67/75 kappa. = 0.57 95% CI 0.3-0.89). Positive percent agreement was 92.3% (60/65); negative percent agreement was 70% (7/10). H1N1 pdm09 identified: 42.6% (32/75) and 54.7% (41/75) by PLEX-ID and CDC RT-PCR, respectively. H3N2 identified: 29.3% (22/75) and 32% (24/75) of samples by PLEX-ID and CDC RT-PCR, respectively. Negatives identified: 16% (12/75) and 13.3% (10/75), by PLEX-ID and CDC RT-PCR respectively. For influenza viruses identified as H1N1 pdm09, Influenza A virus A/NEW YORK/15/2009(H1N1 pdm09) was the most prevalent genotype at 50% (16/32), followed by A/CALIFORNIA/05/2009(H1N1 pdm09) at 18.2% (6/32). Updated assay plates containing additional primers designed for H1N1 pdm09 HA and NA genes were utilized for this evaluation. Among H1N1 pdm09 samples, the HA gene was conserved in 96.9% (31/32) of samples. The NA gene was conserved in 96.9% (31/32).
AB - A comparison study was performed between the PLEX-ID and the CDC RT-PCR method for the detection and identification of Influenza A viruses using nasopharyngeal samples (N= 75) collected between January and May 2011. Overall agreement was 89.3% (67/75 kappa. = 0.57 95% CI 0.3-0.89). Positive percent agreement was 92.3% (60/65); negative percent agreement was 70% (7/10). H1N1 pdm09 identified: 42.6% (32/75) and 54.7% (41/75) by PLEX-ID and CDC RT-PCR, respectively. H3N2 identified: 29.3% (22/75) and 32% (24/75) of samples by PLEX-ID and CDC RT-PCR, respectively. Negatives identified: 16% (12/75) and 13.3% (10/75), by PLEX-ID and CDC RT-PCR respectively. For influenza viruses identified as H1N1 pdm09, Influenza A virus A/NEW YORK/15/2009(H1N1 pdm09) was the most prevalent genotype at 50% (16/32), followed by A/CALIFORNIA/05/2009(H1N1 pdm09) at 18.2% (6/32). Updated assay plates containing additional primers designed for H1N1 pdm09 HA and NA genes were utilized for this evaluation. Among H1N1 pdm09 samples, the HA gene was conserved in 96.9% (31/32) of samples. The NA gene was conserved in 96.9% (31/32).
KW - Genotyping
KW - H1N1 pdm09
KW - Influenza A virus
KW - PLEX-ID
UR - http://www.scopus.com/inward/record.url?scp=84880014343&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880014343&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2013.06.006
DO - 10.1016/j.jviromet.2013.06.006
M3 - Article
C2 - 23764420
AN - SCOPUS:84880014343
SN - 0166-0934
VL - 193
SP - 173
EP - 176
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1
ER -