Evaluation of the Pan Influenza detection kit utilizing the PLEX-ID and influenza samples from the 2011 respiratory season

Luis A. Murillo, Justin Hardick, Kevin Jeng, Charlotte A. Gaydos

Research output: Contribution to journalArticle

Abstract

A comparison study was performed between the PLEX-ID and the CDC RT-PCR method for the detection and identification of Influenza A viruses using nasopharyngeal samples (N= 75) collected between January and May 2011. Overall agreement was 89.3% (67/75 kappa. = 0.57 95% CI 0.3-0.89). Positive percent agreement was 92.3% (60/65); negative percent agreement was 70% (7/10). H1N1 pdm09 identified: 42.6% (32/75) and 54.7% (41/75) by PLEX-ID and CDC RT-PCR, respectively. H3N2 identified: 29.3% (22/75) and 32% (24/75) of samples by PLEX-ID and CDC RT-PCR, respectively. Negatives identified: 16% (12/75) and 13.3% (10/75), by PLEX-ID and CDC RT-PCR respectively. For influenza viruses identified as H1N1 pdm09, Influenza A virus A/NEW YORK/15/2009(H1N1 pdm09) was the most prevalent genotype at 50% (16/32), followed by A/CALIFORNIA/05/2009(H1N1 pdm09) at 18.2% (6/32). Updated assay plates containing additional primers designed for H1N1 pdm09 HA and NA genes were utilized for this evaluation. Among H1N1 pdm09 samples, the HA gene was conserved in 96.9% (31/32) of samples. The NA gene was conserved in 96.9% (31/32).

Original languageEnglish (US)
Pages (from-to)173-176
Number of pages4
JournalJournal of Virological Methods
Volume193
Issue number1
DOIs
StatePublished - Oct 1 2013

Keywords

  • Genotyping
  • H1N1 pdm09
  • Influenza A virus
  • PLEX-ID

ASJC Scopus subject areas

  • Virology

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