Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples

F. Qadri, J. A K Hasan, J. Hossain, A. Chowdhury, Y. Ara Begum, T. Azim, L. Loomis, R. B. Sack, M. J. Albert

Research output: Contribution to journalArticle

Abstract

A monoclonal antibody-based test, Bengal SMART, was developed for rapid detection of Vibrio cholerae O139 synonym Bengal directly from stool specimens. The test, which takes about 15 min to complete, was used to screen 189 diarrheal stool specimens. The results were compared with those of a monoclonal antibody-based coagglutination test (COAT) and the conventional culture methods used as the 'gold standard' for detection of V. cholerae O139. The Bengal SMART test showed a sensitivity of 100% and a specificity of 97% in comparison with the gold standard. It also fared better than COAT, which had a sensitivity of 96% for rapid detection of V. cholerae O139 synonym Bengal. These results show that Bengal SMART is suitable for use in field settings for rapid diagnosis of cholera caused by V. cholerae O139.

Original languageEnglish (US)
Pages (from-to)732-734
Number of pages3
JournalJournal of Clinical Microbiology
Volume33
Issue number3
StatePublished - 1995
Externally publishedYes

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Vibrio cholerae O139
Monoclonal Antibodies
Cholera

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Qadri, F., Hasan, J. A. K., Hossain, J., Chowdhury, A., Ara Begum, Y., Azim, T., ... Albert, M. J. (1995). Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples. Journal of Clinical Microbiology, 33(3), 732-734.

Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples. / Qadri, F.; Hasan, J. A K; Hossain, J.; Chowdhury, A.; Ara Begum, Y.; Azim, T.; Loomis, L.; Sack, R. B.; Albert, M. J.

In: Journal of Clinical Microbiology, Vol. 33, No. 3, 1995, p. 732-734.

Research output: Contribution to journalArticle

Qadri, F, Hasan, JAK, Hossain, J, Chowdhury, A, Ara Begum, Y, Azim, T, Loomis, L, Sack, RB & Albert, MJ 1995, 'Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples', Journal of Clinical Microbiology, vol. 33, no. 3, pp. 732-734.
Qadri, F. ; Hasan, J. A K ; Hossain, J. ; Chowdhury, A. ; Ara Begum, Y. ; Azim, T. ; Loomis, L. ; Sack, R. B. ; Albert, M. J. / Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples. In: Journal of Clinical Microbiology. 1995 ; Vol. 33, No. 3. pp. 732-734.
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AU - Hasan, J. A K

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AU - Chowdhury, A.

AU - Ara Begum, Y.

AU - Azim, T.

AU - Loomis, L.

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AU - Albert, M. J.

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