Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples

F. Qadri; J. A.K. Hasan; J. Hossain; A. Chowdhury; Y. Ara Begum; T. Azim; L. Loomis; R. B. Sack; M. J. Albert

doi:10.1128/jcm.33.3.732-734.1995

Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples

F. Qadri, J. A.K. Hasan, J. Hossain, A. Chowdhury, Y. Ara Begum, T. Azim, L. Loomis, R. B. Sack, M. J. Albert

Research output: Contribution to journal › Comment/debate › peer-review

42 Scopus citations

Abstract

A monoclonal antibody-based test, Bengal SMART, was developed for rapid detection of Vibrio cholerae O139 synonym Bengal directly from stool specimens. The test, which takes about 15 min to complete, was used to screen 189 diarrheal stool specimens. The results were compared with those of a monoclonal antibody-based coagglutination test (COAT) and the conventional culture methods used as the 'gold standard' for detection of V. cholerae O139. The Bengal SMART test showed a sensitivity of 100% and a specificity of 97% in comparison with the gold standard. It also fared better than COAT, which had a sensitivity of 96% for rapid detection of V. cholerae O139 synonym Bengal. These results show that Bengal SMART is suitable for use in field settings for rapid diagnosis of cholera caused by V. cholerae O139.

Original language	English (US)
Pages (from-to)	732-734
Number of pages	3
Journal	Journal of clinical microbiology
Volume	33
Issue number	3
DOIs	https://doi.org/10.1128/jcm.33.3.732-734.1995
State	Published - 1995
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.33.3.732-734.1995

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title = "Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples",

abstract = "A monoclonal antibody-based test, Bengal SMART, was developed for rapid detection of Vibrio cholerae O139 synonym Bengal directly from stool specimens. The test, which takes about 15 min to complete, was used to screen 189 diarrheal stool specimens. The results were compared with those of a monoclonal antibody-based coagglutination test (COAT) and the conventional culture methods used as the 'gold standard' for detection of V. cholerae O139. The Bengal SMART test showed a sensitivity of 100% and a specificity of 97% in comparison with the gold standard. It also fared better than COAT, which had a sensitivity of 96% for rapid detection of V. cholerae O139 synonym Bengal. These results show that Bengal SMART is suitable for use in field settings for rapid diagnosis of cholera caused by V. cholerae O139.",

author = "F. Qadri and Hasan, {J. A.K.} and J. Hossain and A. Chowdhury and {Ara Begum}, Y. and T. Azim and L. Loomis and Sack, {R. B.} and Albert, {M. J.}",

year = "1995",

doi = "10.1128/jcm.33.3.732-734.1995",

language = "English (US)",

volume = "33",

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T1 - Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples

AU - Qadri, F.

AU - Hasan, J. A.K.

AU - Hossain, J.

AU - Chowdhury, A.

AU - Ara Begum, Y.

AU - Azim, T.

AU - Loomis, L.

AU - Sack, R. B.

AU - Albert, M. J.

PY - 1995

Y1 - 1995

N2 - A monoclonal antibody-based test, Bengal SMART, was developed for rapid detection of Vibrio cholerae O139 synonym Bengal directly from stool specimens. The test, which takes about 15 min to complete, was used to screen 189 diarrheal stool specimens. The results were compared with those of a monoclonal antibody-based coagglutination test (COAT) and the conventional culture methods used as the 'gold standard' for detection of V. cholerae O139. The Bengal SMART test showed a sensitivity of 100% and a specificity of 97% in comparison with the gold standard. It also fared better than COAT, which had a sensitivity of 96% for rapid detection of V. cholerae O139 synonym Bengal. These results show that Bengal SMART is suitable for use in field settings for rapid diagnosis of cholera caused by V. cholerae O139.

AB - A monoclonal antibody-based test, Bengal SMART, was developed for rapid detection of Vibrio cholerae O139 synonym Bengal directly from stool specimens. The test, which takes about 15 min to complete, was used to screen 189 diarrheal stool specimens. The results were compared with those of a monoclonal antibody-based coagglutination test (COAT) and the conventional culture methods used as the 'gold standard' for detection of V. cholerae O139. The Bengal SMART test showed a sensitivity of 100% and a specificity of 97% in comparison with the gold standard. It also fared better than COAT, which had a sensitivity of 96% for rapid detection of V. cholerae O139 synonym Bengal. These results show that Bengal SMART is suitable for use in field settings for rapid diagnosis of cholera caused by V. cholerae O139.

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JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

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ER -

Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples

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