TY - JOUR
T1 - Evaluation of the interactions between multiwalled carbon nanotubes and caco-2 cells
AU - Clark, Katherine A.
AU - O'Driscoll, Cliona
AU - Cooke, Carol A.
AU - Smith, Bill A.
AU - Wepasnick, Kevin
AU - Fairbrother, D. Howard
AU - Lees, Peter S.J.
AU - Bressler, Joseph P.
N1 - Funding Information:
Received 16 February 2011; accepted 28 April 2011. Funding for this research was provided by the National Institute of Occupational Safety and Health Pilot Project Research Training Program (Johns Hopkins Bloomberg School of Public Health number T42 OH00842428) and the Institute for NanoBioTechnology at the Johns Hopkins University. We thank Michelle Husain for her assistance in TEM imaging. Address correspondence to Joseph P. Bressler, Kennedy Krieger Institute, 707 North Broadway, Baltimore MD 21215, USA. E-mail: bressler@kennedykrieger.org
PY - 2012
Y1 - 2012
N2 - The aim of this study was to determine whether multiwalled carbon nanotubes (MWNCT) are taken up by and are toxic to human intestinal enterocytes using the Caco-2 cell model. Caco-2 cells were exposed to 50 μg/ml MWCNT (oxidized or pristine) for 24 h, and experiments were repeated in the presence of 2.5 mg/L natural organic matter. Cells display yed many ofthe properties that characterize enterocytes, such as apical microvilli, basolateral basementmembrane, and glycogen. The cell monolayers also displayed tight junctions and electricalresistance. Exposure to pristine and oxidized MWCNT, with or without natural rganic matter,did not markedly affect viability, which was assessed by measuring activity of relea sed lactatedehydrogenase (LDH) and staining with propidium iodide. Ultrastructural ana lysis revealedsome damage to microvilli colocalized with the MWCNT; however, neither typ of MWCNT was taken up by Caco-2 cells. In contrast, pristine and oxidized MWCNT were taken up by the macrophage RAW 264.7 line. Our study suggests that intestinal enterocytes cells do not take up MWCNT.
AB - The aim of this study was to determine whether multiwalled carbon nanotubes (MWNCT) are taken up by and are toxic to human intestinal enterocytes using the Caco-2 cell model. Caco-2 cells were exposed to 50 μg/ml MWCNT (oxidized or pristine) for 24 h, and experiments were repeated in the presence of 2.5 mg/L natural organic matter. Cells display yed many ofthe properties that characterize enterocytes, such as apical microvilli, basolateral basementmembrane, and glycogen. The cell monolayers also displayed tight junctions and electricalresistance. Exposure to pristine and oxidized MWCNT, with or without natural rganic matter,did not markedly affect viability, which was assessed by measuring activity of relea sed lactatedehydrogenase (LDH) and staining with propidium iodide. Ultrastructural ana lysis revealedsome damage to microvilli colocalized with the MWCNT; however, neither typ of MWCNT was taken up by Caco-2 cells. In contrast, pristine and oxidized MWCNT were taken up by the macrophage RAW 264.7 line. Our study suggests that intestinal enterocytes cells do not take up MWCNT.
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U2 - 10.1080/15287394.2011.589105
DO - 10.1080/15287394.2011.589105
M3 - Article
C2 - 22047161
AN - SCOPUS:81255147881
VL - 75
SP - 25
EP - 35
JO - Journal of Toxicology and Environmental Health - Part A: Current Issues
JF - Journal of Toxicology and Environmental Health - Part A: Current Issues
SN - 1528-7394
IS - 1
ER -