Evaluation of the effects of disinfectants on rotavirus RNA and infectivity by the polymerase chain reaction and cell-culture methods

C. K. Ojeh, T. M. Cusack, Robert H Yolken

Research output: Contribution to journalArticle

Abstract

Rotaviruses have been linked to outbreaks of acute gastroenteritis of children in day-care centres and hospital paediatric wards. There is, therefore, the need for monitoring effective decontamination of such environments. We have evaluated the effects of seven different methods of disinfection/inactivation (four chemical and three physical) on rotavirus using the PCR and cell-culture methods. We observed that 6% H2O2, 2500 ppm chlorine, an ethano-phenolic disinfectant, u.v. irradiation and heat completely destroyed the infectivity of rotavirus as well as RNA amplifiable by PCR. On the ther hand, treatment with 80% ethanol resulted in the loss of infectivity despite the fact that RNA was still amplifiable. Rotavirus subjected to drying over a 24 h period still retained amplifiable RNA but infectivity was reduced by 100-fold when compared to the control. This study demonstrated an agreement between PCR and cell-culture monitoring systems, PCR is a more rapid and sensitive assay.

Original languageEnglish (US)
Pages (from-to)341-346
Number of pages6
JournalMolecular and Cellular Probes
Volume9
Issue number5
DOIs
StatePublished - 1995

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Disinfectants
Rotavirus
DNA-Directed RNA Polymerases
Cell Culture Techniques
Polymerase Chain Reaction
RNA
Child Day Care Centers
Pediatric Hospitals
Decontamination
Chlorine
Disinfection
Gastroenteritis
Disease Outbreaks
Ethanol
Hand
Hot Temperature

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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Evaluation of the effects of disinfectants on rotavirus RNA and infectivity by the polymerase chain reaction and cell-culture methods. / Ojeh, C. K.; Cusack, T. M.; Yolken, Robert H.

In: Molecular and Cellular Probes, Vol. 9, No. 5, 1995, p. 341-346.

Research output: Contribution to journalArticle

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