Evaluation of the BD vacutainer plus urine C&S preservative tubes compared with nonpreservative urine samples stored at 4°C and room temperature

Stephen W. Eisinger, Matthew Schwartz, Lisa Dam, Stefan Riedel

Research output: Contribution to journalArticle

Abstract

Objectives: The stability of urine specimens submitted for culture remains a challenge for many laboratories because of delays in specimen transport. We evaluated the usefulness of BD Vacutainer Plus Urine C&S Preservative Tube in ensuring specimen stability. Methods: Clinical urine specimens collected in sterile collection cups (n = 110) were plated onto sheep blood and MacConkey agar following standard laboratory procedures guidelines. Thereafter, specimens were divided into 3 storage conditions: nonpreservative, refrigerated; nonpreservative, room temperature (RT); BD Vacutainer Plus Urine C&S Preservative Tube, RT. For each sample type, additional cultures were set up at 2, 4, 24, and 48 hours. Results: Initially, 18 specimens had no growth, 32 showed mixed skin flora, and 60 yielded at least 1 uropathogen. Increased colony counts of uropathogens were observed for nonpreserved urine samples stored at RT; these changes were statistically significant. Minor differences between refrigerated urine samples and BD Vacutainer Plus Urine C&S Preservative Tube samples were seen but were not statistically significant. Conclusions: The use of preservative-containing collection tubes is desirable to ensure specimen stability when prompt processing or refrigeration is not feasible.

Original languageEnglish (US)
Pages (from-to)306-313
Number of pages8
JournalAmerican journal of clinical pathology
Volume140
Issue number3
DOIs
StatePublished - Sep 1 2013

Keywords

  • BD vacutainer plus urine C&S preservative tube
  • Specimen stability
  • Specimen transport
  • Urine culture
  • Urine specimen storage

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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