Evaluation of the antimicrobial effects of several isothiocyanates on Helicobacter pylori

Xavier Haristoy; Jed W. Fahey; Isabelle Scholtus; Alain Lozniewski

doi:10.1055/s-2005-864098

Evaluation of the antimicrobial effects of several isothiocyanates on Helicobacter pylori

Xavier Haristoy, Jed W. Fahey, Isabelle Scholtus, Alain Lozniewski

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

The antibacterial activity of sulforaphane [4-(methylsulfinyl)butyl isothiocyanate] has been previously described. We have analysed the activities of 12 isothiocyanates (ITC) including sulphoraphane on 25 strains of Helicobacter pylori using an agar dilution assay. In addition, bactericidal effects against H. pylon were determined for the 6 most active ITCs, both directly and against intracellular bacteria in cultured human epithelial (HEp-2) cells. The MIC₉₀ values for these ITCs ranged between 4 and 32 μg/mL and four of the most potent compounds exhibited bactericidal activity against both extra- and intracellular bacteria. Overall, our data indicate that ITCs have a potent antibacterial effect against H. pylori and these naturally occurring phytochemicals might have potential as novel therapeutic agents for H. pylori eradication.

Original language	English (US)
Pages (from-to)	326-330
Number of pages	5
Journal	Planta Medica
Volume	71
Issue number	4
DOIs	https://doi.org/10.1055/s-2005-864098
State	Published - 2005
Externally published	Yes

Keywords

Helicobacter pylon
Isothiocyanate
Minimal inhibitory concentration
Time-to-kill assay

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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10.1055/s-2005-864098

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title = "Evaluation of the antimicrobial effects of several isothiocyanates on Helicobacter pylori",

abstract = "The antibacterial activity of sulforaphane [4-(methylsulfinyl)butyl isothiocyanate] has been previously described. We have analysed the activities of 12 isothiocyanates (ITC) including sulphoraphane on 25 strains of Helicobacter pylori using an agar dilution assay. In addition, bactericidal effects against H. pylon were determined for the 6 most active ITCs, both directly and against intracellular bacteria in cultured human epithelial (HEp-2) cells. The MIC90 values for these ITCs ranged between 4 and 32 μg/mL and four of the most potent compounds exhibited bactericidal activity against both extra- and intracellular bacteria. Overall, our data indicate that ITCs have a potent antibacterial effect against H. pylori and these naturally occurring phytochemicals might have potential as novel therapeutic agents for H. pylori eradication.",

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doi = "10.1055/s-2005-864098",

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T1 - Evaluation of the antimicrobial effects of several isothiocyanates on Helicobacter pylori

AU - Haristoy, Xavier

AU - Fahey, Jed W.

AU - Scholtus, Isabelle

AU - Lozniewski, Alain

PY - 2005

Y1 - 2005

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AB - The antibacterial activity of sulforaphane [4-(methylsulfinyl)butyl isothiocyanate] has been previously described. We have analysed the activities of 12 isothiocyanates (ITC) including sulphoraphane on 25 strains of Helicobacter pylori using an agar dilution assay. In addition, bactericidal effects against H. pylon were determined for the 6 most active ITCs, both directly and against intracellular bacteria in cultured human epithelial (HEp-2) cells. The MIC90 values for these ITCs ranged between 4 and 32 μg/mL and four of the most potent compounds exhibited bactericidal activity against both extra- and intracellular bacteria. Overall, our data indicate that ITCs have a potent antibacterial effect against H. pylori and these naturally occurring phytochemicals might have potential as novel therapeutic agents for H. pylori eradication.

KW - Helicobacter pylon

KW - Isothiocyanate

KW - Minimal inhibitory concentration

KW - Time-to-kill assay

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Evaluation of the antimicrobial effects of several isothiocyanates on Helicobacter pylori

Abstract

Keywords

ASJC Scopus subject areas

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