TY - JOUR
T1 - Evaluation of temperature-sensitive, indocyanine green-encapsulating micelles for noninvasive near-infrared tumor imaging
AU - Kim, Tae Hee
AU - Chen, Yongping
AU - Mount, Christopher W.
AU - Gombotz, Wayne R.
AU - Li, Xingde
AU - Pun, Suzie H.
N1 - Funding Information:
This work was supported by the funds from the Washington Technology Center and Omeros Corporation (Pun and Li), and in part by the National Institutes of Health (Grant No. R01 CA120480-Li). Xenogen Spectrum imaging was conducted through the Center for Intracellular Delivery of Biologics, funded by Washington State Life Sciences Discovery Fund Grant 2496490.
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2010/9
Y1 - 2010/9
N2 - Purpose: Indocyanine green (ICG), an FDA-approved near infrared (NIR) dye, has potential application as a contrast agent for tumor detection. Because ICG binds strongly to plasma proteins and exhibits aqueous, photo, and thermal instability, its current applications are largely limited to monitoring blood flow. To address these issues, ICG was encapsulated and stabilized within polymeric micelles formed from the thermo-sensitive block copolymer Pluronic F-127, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide), to increase the stability and circulation time of ICG. Methods: ICG-loaded Pluronic micelles were prepared at various concentrations of Pluronic and ICG and characterized by determining particle sizes, dye loading efficiency, and the kinetics of dye degradation. Förster resonance energy transfer spectroscopy was employed to monitor the stability of Pluronic micelles in physiological solutions. The plasma clearance kinetics and biodistribution of ICG-loaded micelles was also determined after intravenous delivery to CT-26 colon carcinoma tumor-bearing mice, and NIR whole-body imaging was performed for tumor detection. Results: The Pluronic F-127 micelles showed efficient ICG loading, small size, stabilized ICG fluorescence, and prolonged circulation in vivo. Solid tumors in mice were specifically visualized after intravenous administration of ICG-loaded micelles. Conclusions: These materials are therefore promising formulations for noninvasive NIR tumor imaging applications.
AB - Purpose: Indocyanine green (ICG), an FDA-approved near infrared (NIR) dye, has potential application as a contrast agent for tumor detection. Because ICG binds strongly to plasma proteins and exhibits aqueous, photo, and thermal instability, its current applications are largely limited to monitoring blood flow. To address these issues, ICG was encapsulated and stabilized within polymeric micelles formed from the thermo-sensitive block copolymer Pluronic F-127, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide), to increase the stability and circulation time of ICG. Methods: ICG-loaded Pluronic micelles were prepared at various concentrations of Pluronic and ICG and characterized by determining particle sizes, dye loading efficiency, and the kinetics of dye degradation. Förster resonance energy transfer spectroscopy was employed to monitor the stability of Pluronic micelles in physiological solutions. The plasma clearance kinetics and biodistribution of ICG-loaded micelles was also determined after intravenous delivery to CT-26 colon carcinoma tumor-bearing mice, and NIR whole-body imaging was performed for tumor detection. Results: The Pluronic F-127 micelles showed efficient ICG loading, small size, stabilized ICG fluorescence, and prolonged circulation in vivo. Solid tumors in mice were specifically visualized after intravenous administration of ICG-loaded micelles. Conclusions: These materials are therefore promising formulations for noninvasive NIR tumor imaging applications.
KW - indocyanine green
KW - micelle
KW - near-infrared tumor imaging
KW - stability
KW - thermo-sensitivity
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U2 - 10.1007/s11095-010-0190-y
DO - 10.1007/s11095-010-0190-y
M3 - Article
C2 - 20568000
AN - SCOPUS:77955466654
SN - 0724-8741
VL - 27
SP - 1900
EP - 1913
JO - Pharmaceutical Research
JF - Pharmaceutical Research
IS - 9
ER -