Evaluation of 51Cr as a Leucocyte Label

Jerry L. Spivak, Seymour Perry

Research output: Contribution to journalArticle

Abstract

Summary. A double label technique has been developed to compare the behaviour of 51Cr labelled leucocytes with DF32P labelled leucocytes. Whole blood is divided into two aliquots and the leucocytes in each are labelled with a different isotope. After infusion of labelled cells, the radioactivity of each isotope can be determined simultaneously on the same blood sample using liquid scintillation counting. In studies of seven patients with solid tumours and one with leukaemia, the intravascular disappearance of autologous leucocytes labelled with 51Cr and DF32P appeared similar in five of the eight patients during the initial 6 hr following infusion. Thereafter in all but one instance, 51Cr labelled leucocytes had slightly higher specific activities. 51Cr labelled lymphocytes and DF32P labelled lymphocytes also behaved in the same manner. It is concluded that51Cr is a suitable leucocyte label and may be substituted for DF32P under certain circumstances.

Original languageEnglish (US)
Pages (from-to)321-330
Number of pages10
JournalBritish journal of haematology
Volume25
Issue number3
DOIs
StatePublished - Sep 1973

ASJC Scopus subject areas

  • Hematology

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