Evaluation of serum and seminal plasma markers in the diagnosis of canine prostatic disorders.

F. W. Bell, J. S. Klausner, D. W. Hayden, E. M. Lund, B. B. Liebenstein, D. A. Feeney, S. D. Johnston, J. L. Shivers, C. M. Ewing, William B Isaacs

Research output: Contribution to journalArticle

Abstract

Serum and seminal plasma concentrations or activities of acid phosphatase (AP), prostate specific antigen (PSA), and canine prostate specific esterase (CPSE) were measured in normal dogs, dogs with benign prostatic hyperplasia (BPH), dogs with bacterial prostatitis, and dogs with prostatic carcinoma to determine if these assays would be of value in differentiating dogs with prostatic carcinoma from normal dogs, and dogs with other prostatic disorders. In addition, tissue sections of prostatic adenocarcinomas were stained with antiprostatic AP, anti-CPSE, and anti-PSA antibodies to determine if these would be suitable immunohistochemical markers of prostatic carcinoma. Prostate-specific antigen was not detected in canine serum or seminal plasma. Serum and seminal AP activities did not differ significantly between normal dogs and those with prostatic diseases, or among dogs with different prostatic disorders. Serum CPSE activities were significantly higher in dogs with BPH than in normal dogs. Mean serum CPSE activities in dogs with BPH, bacterial prostatitis, and prostatic carcinoma were not significantly different from each other. Slight to moderate immunohistochemical staining of canine prostatic adenocarcinomas was noted for prostatic AP and PSA; most tumors did not stain for CPSE. These results show that proteins of prostatic origin appear in the serum of dogs as a result of prostatic pathology, especially BPH. Canine prostatic adenocarcinoma does not appear to be associated with significant increases in CPSE or AP activities, possibly because of down-regulation of these enzymes by prostatic carcinoma cells. It is also possible that failure to detect significant differences resulted from limited statistical power for some groups and pairwise analyses because of the small number of dogs evaluated.

Original languageEnglish (US)
Pages (from-to)149-153
Number of pages5
JournalJournal of veterinary internal medicine / American College of Veterinary Internal Medicine
Volume9
Issue number3
StatePublished - May 1995

Fingerprint

seminal plasma
Semen
Canidae
Dogs
dogs
Serum
Esterases
Prostate
Prostatic Hyperplasia
Prostate-Specific Antigen
Acid Phosphatase
prostate-specific antigen
esterases
Carcinoma
acid phosphatase
carcinoma
Prostatitis
Adenocarcinoma
hyperplasia
adenocarcinoma

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of serum and seminal plasma markers in the diagnosis of canine prostatic disorders. / Bell, F. W.; Klausner, J. S.; Hayden, D. W.; Lund, E. M.; Liebenstein, B. B.; Feeney, D. A.; Johnston, S. D.; Shivers, J. L.; Ewing, C. M.; Isaacs, William B.

In: Journal of veterinary internal medicine / American College of Veterinary Internal Medicine, Vol. 9, No. 3, 05.1995, p. 149-153.

Research output: Contribution to journalArticle

Bell, FW, Klausner, JS, Hayden, DW, Lund, EM, Liebenstein, BB, Feeney, DA, Johnston, SD, Shivers, JL, Ewing, CM & Isaacs, WB 1995, 'Evaluation of serum and seminal plasma markers in the diagnosis of canine prostatic disorders.', Journal of veterinary internal medicine / American College of Veterinary Internal Medicine, vol. 9, no. 3, pp. 149-153.
Bell, F. W. ; Klausner, J. S. ; Hayden, D. W. ; Lund, E. M. ; Liebenstein, B. B. ; Feeney, D. A. ; Johnston, S. D. ; Shivers, J. L. ; Ewing, C. M. ; Isaacs, William B. / Evaluation of serum and seminal plasma markers in the diagnosis of canine prostatic disorders. In: Journal of veterinary internal medicine / American College of Veterinary Internal Medicine. 1995 ; Vol. 9, No. 3. pp. 149-153.
@article{0daefac77cdf4ccaa4b512400fae8eb2,
title = "Evaluation of serum and seminal plasma markers in the diagnosis of canine prostatic disorders.",
abstract = "Serum and seminal plasma concentrations or activities of acid phosphatase (AP), prostate specific antigen (PSA), and canine prostate specific esterase (CPSE) were measured in normal dogs, dogs with benign prostatic hyperplasia (BPH), dogs with bacterial prostatitis, and dogs with prostatic carcinoma to determine if these assays would be of value in differentiating dogs with prostatic carcinoma from normal dogs, and dogs with other prostatic disorders. In addition, tissue sections of prostatic adenocarcinomas were stained with antiprostatic AP, anti-CPSE, and anti-PSA antibodies to determine if these would be suitable immunohistochemical markers of prostatic carcinoma. Prostate-specific antigen was not detected in canine serum or seminal plasma. Serum and seminal AP activities did not differ significantly between normal dogs and those with prostatic diseases, or among dogs with different prostatic disorders. Serum CPSE activities were significantly higher in dogs with BPH than in normal dogs. Mean serum CPSE activities in dogs with BPH, bacterial prostatitis, and prostatic carcinoma were not significantly different from each other. Slight to moderate immunohistochemical staining of canine prostatic adenocarcinomas was noted for prostatic AP and PSA; most tumors did not stain for CPSE. These results show that proteins of prostatic origin appear in the serum of dogs as a result of prostatic pathology, especially BPH. Canine prostatic adenocarcinoma does not appear to be associated with significant increases in CPSE or AP activities, possibly because of down-regulation of these enzymes by prostatic carcinoma cells. It is also possible that failure to detect significant differences resulted from limited statistical power for some groups and pairwise analyses because of the small number of dogs evaluated.",
author = "Bell, {F. W.} and Klausner, {J. S.} and Hayden, {D. W.} and Lund, {E. M.} and Liebenstein, {B. B.} and Feeney, {D. A.} and Johnston, {S. D.} and Shivers, {J. L.} and Ewing, {C. M.} and Isaacs, {William B}",
year = "1995",
month = "5",
language = "English (US)",
volume = "9",
pages = "149--153",
journal = "Journal of Veterinary Internal Medicine",
issn = "0891-6640",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Evaluation of serum and seminal plasma markers in the diagnosis of canine prostatic disorders.

AU - Bell, F. W.

AU - Klausner, J. S.

AU - Hayden, D. W.

AU - Lund, E. M.

AU - Liebenstein, B. B.

AU - Feeney, D. A.

AU - Johnston, S. D.

AU - Shivers, J. L.

AU - Ewing, C. M.

AU - Isaacs, William B

PY - 1995/5

Y1 - 1995/5

N2 - Serum and seminal plasma concentrations or activities of acid phosphatase (AP), prostate specific antigen (PSA), and canine prostate specific esterase (CPSE) were measured in normal dogs, dogs with benign prostatic hyperplasia (BPH), dogs with bacterial prostatitis, and dogs with prostatic carcinoma to determine if these assays would be of value in differentiating dogs with prostatic carcinoma from normal dogs, and dogs with other prostatic disorders. In addition, tissue sections of prostatic adenocarcinomas were stained with antiprostatic AP, anti-CPSE, and anti-PSA antibodies to determine if these would be suitable immunohistochemical markers of prostatic carcinoma. Prostate-specific antigen was not detected in canine serum or seminal plasma. Serum and seminal AP activities did not differ significantly between normal dogs and those with prostatic diseases, or among dogs with different prostatic disorders. Serum CPSE activities were significantly higher in dogs with BPH than in normal dogs. Mean serum CPSE activities in dogs with BPH, bacterial prostatitis, and prostatic carcinoma were not significantly different from each other. Slight to moderate immunohistochemical staining of canine prostatic adenocarcinomas was noted for prostatic AP and PSA; most tumors did not stain for CPSE. These results show that proteins of prostatic origin appear in the serum of dogs as a result of prostatic pathology, especially BPH. Canine prostatic adenocarcinoma does not appear to be associated with significant increases in CPSE or AP activities, possibly because of down-regulation of these enzymes by prostatic carcinoma cells. It is also possible that failure to detect significant differences resulted from limited statistical power for some groups and pairwise analyses because of the small number of dogs evaluated.

AB - Serum and seminal plasma concentrations or activities of acid phosphatase (AP), prostate specific antigen (PSA), and canine prostate specific esterase (CPSE) were measured in normal dogs, dogs with benign prostatic hyperplasia (BPH), dogs with bacterial prostatitis, and dogs with prostatic carcinoma to determine if these assays would be of value in differentiating dogs with prostatic carcinoma from normal dogs, and dogs with other prostatic disorders. In addition, tissue sections of prostatic adenocarcinomas were stained with antiprostatic AP, anti-CPSE, and anti-PSA antibodies to determine if these would be suitable immunohistochemical markers of prostatic carcinoma. Prostate-specific antigen was not detected in canine serum or seminal plasma. Serum and seminal AP activities did not differ significantly between normal dogs and those with prostatic diseases, or among dogs with different prostatic disorders. Serum CPSE activities were significantly higher in dogs with BPH than in normal dogs. Mean serum CPSE activities in dogs with BPH, bacterial prostatitis, and prostatic carcinoma were not significantly different from each other. Slight to moderate immunohistochemical staining of canine prostatic adenocarcinomas was noted for prostatic AP and PSA; most tumors did not stain for CPSE. These results show that proteins of prostatic origin appear in the serum of dogs as a result of prostatic pathology, especially BPH. Canine prostatic adenocarcinoma does not appear to be associated with significant increases in CPSE or AP activities, possibly because of down-regulation of these enzymes by prostatic carcinoma cells. It is also possible that failure to detect significant differences resulted from limited statistical power for some groups and pairwise analyses because of the small number of dogs evaluated.

UR - http://www.scopus.com/inward/record.url?scp=0029301049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029301049&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 149

EP - 153

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

SN - 0891-6640

IS - 3

ER -