TY - JOUR
T1 - Evaluation of selected lichens from Iceland for cancer chemopreventive and cytotoxic activity
AU - Ingolfsdottir, K.
AU - Sang Kook Lee, Kook Lee
AU - Bhat, K. P.L.
AU - Lee, K.
AU - Chai, H. B.
AU - Kristinsson, H.
AU - Song, L. L.
AU - Gills, J.
AU - Gudmundsdottir, J. Th
AU - Mata-Greenwood, E.
AU - Jang, M. S.
AU - Pezzuto, J. M.
N1 - Funding Information:
Conflict-of-interest disclosure: J.D.G., J.S., and G.A.B. are inventors on a patent (Patent Cooperation Treaty patent application PCT/US18/15918) submitted by The Children’s Hospital of Philadelphia that covers the therapeutic targeting of HRI for hemoglobinopathies. G.A.B holds licensing agreements with Pfizer Inc and Vertex Pharmaceuticals and has received research funding from Pfizer Inc and served as a consultant for Fulcrum Therapeutics. The remaining authors declare no competing financial interests.
Funding Information:
This work was supported by National Institutes of Health, National Heart, Lung, and Blood Institute grants R01HL119479 (G.A.B.) and T32HL007439 (S.A.P); Doris Duke Charitable Foundation Physician Scientist Fellowship grant 2020062 (S.A.P.); the St Jude Children’s Research Hospital Collaborative Research Consortium on Novel Gene Therapies for Sickle Cell Disease; research funding from Pfizer Inc (G.A.B); and a generous gift from the DiGaetano family.
PY - 2000
Y1 - 2000
N2 - Cancer chemopreventive effects of organic extracts from 29 species of lichens collected in Iceland were evaluated using a panel of in vitro bioassays whereby extracts were tested for potential to induce quinone reductase (QR) and differentiation of human promye-locytic leukemia (HL-60) cells, inhibit cyclooxygenase-1 (COX-1), phorbol ester-induced ornithine decarboxylase (ODC), aromatase and sulfatase, as well as for antioxidant, estrogenic/anti-estrogenic and antiproliferative activity. In addition, the extracts were tested for cytotoxiciry against 12 cancer cell lines. The most significant results were exhibited by extracts from Xanthoria elegans and Alectoria nigricans, which respectively, induced QR activity (concentration to double activity = 4.8 μg/ml) and inhibited phorbol ester-induced ODC activity with mouse 308 cells in culture (1C50 = 2.6 μg/ml). Moderate inhibition of [3H]thymidine incorporation with HL-60 cells was exhibited by the Peltigera leucophlebia extract. Several extracts prevented estrogen formation from estrogen precursors by inhibiting the enzymatic activities of aromatase (Sphaerophorus globosus, Cetrariella delisei, Melanelia hepatizon) and sulfatase (Cladonia gracilis, Sphaerophorus fragilis, S. globosus). None of the extracts demonstrated significant cytotoxic effects with selected cell lines.
AB - Cancer chemopreventive effects of organic extracts from 29 species of lichens collected in Iceland were evaluated using a panel of in vitro bioassays whereby extracts were tested for potential to induce quinone reductase (QR) and differentiation of human promye-locytic leukemia (HL-60) cells, inhibit cyclooxygenase-1 (COX-1), phorbol ester-induced ornithine decarboxylase (ODC), aromatase and sulfatase, as well as for antioxidant, estrogenic/anti-estrogenic and antiproliferative activity. In addition, the extracts were tested for cytotoxiciry against 12 cancer cell lines. The most significant results were exhibited by extracts from Xanthoria elegans and Alectoria nigricans, which respectively, induced QR activity (concentration to double activity = 4.8 μg/ml) and inhibited phorbol ester-induced ODC activity with mouse 308 cells in culture (1C50 = 2.6 μg/ml). Moderate inhibition of [3H]thymidine incorporation with HL-60 cells was exhibited by the Peltigera leucophlebia extract. Several extracts prevented estrogen formation from estrogen precursors by inhibiting the enzymatic activities of aromatase (Sphaerophorus globosus, Cetrariella delisei, Melanelia hepatizon) and sulfatase (Cladonia gracilis, Sphaerophorus fragilis, S. globosus). None of the extracts demonstrated significant cytotoxic effects with selected cell lines.
KW - Aromatase
KW - Cancer chemoprevention
KW - Cytotoxicity
KW - HL-60 cell differentiation
KW - Icelandic plants
KW - Lichens
KW - Ornithine decarboxylase
KW - Quinone reductase
KW - Sulfatase
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U2 - 10.1076/1388-0209(200009)3841-AFT313
DO - 10.1076/1388-0209(200009)3841-AFT313
M3 - Article
C2 - 21214483
AN - SCOPUS:0033748375
SN - 1388-0209
VL - 38
SP - 313
EP - 317
JO - Pharmaceutical Biology
JF - Pharmaceutical Biology
IS - 4
ER -