Evaluation of Rapid Diagnostic Tests for Typhoid Fever

Sonja J. Olsen, Jim Pruckler, William Bibb, Nguyen Thi My Thanh, Tran My Trinh, Nguyen Thi Minh, Sumathi Sivapalasingam, Amita Gupta, Phan Thu Phuong, Nguyen Tran Chinh, Nguyen Vinh Chau, Phung Dac Cam, Eric D. Mintz

Research output: Contribution to journalArticle

Abstract

Laboratory diagnosis of typhoid fever requires isolation and identification of Salmonella enterica serotype Typhi. In many areas where this disease is endemic, laboratory capability is limited. Recent advances in molecular immunology have led to the identification of sensitive and specific markers for typhoid fever and technology to manufacture practical and inexpensive kits for their rapid detection. We evaluated three commercial kits for serologic diagnosis of typhoid fever. Patients presenting with ≥ 4 days of fever were enrolled at two hospitals in Southern Vietnam. Cases were patients with serotype Typhi isolated from blood samples, and controls were patients with other laboratory-confirmed illnesses. Serotype Typhi isolates were confirmed and tested for antimicrobial susceptibility at the Pasteur Institute in Ho Chi Minh City. The Widal test was run at the hospitals and the Pasteur Institute. Sera were shipped frozen to the Centers for Disease Control and Prevention and tested by using Multi-Test Dip-S-Ticks, TyphiDot, and TUBEX to detect immunoglobulin G (IgG), IgG and IgM, and IgM, respectively. Package insert protocol instructions were followed. We enrolled 59 patients and 21 controls. The sensitivity and specificity findings were as follows: 89 and 53% for Multi-Test Dip-S-Ticks, 79 and 89% for TyphiDot, 78 and 89% for TUBEX, and 64 and 76% for Widal testing in hospitals and 61% and 100% for Widal testing at the Pasteur Institute. For all assays, the sensitivity was highest in the second week of illness. The Widal test was insensitive and displayed interoperator variability. Two rapid kits, TyphiDot and TUBEX, demonstrated promising results.

Original languageEnglish (US)
Pages (from-to)1885-1889
Number of pages5
JournalJournal of Clinical Microbiology
Volume42
Issue number5
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

Typhoid Fever
Routine Diagnostic Tests
Ticks
Immunoglobulin M
Immunoglobulin G
Product Labeling
Endemic Diseases
Salmonella enterica
Vietnam
Clinical Laboratory Techniques
Centers for Disease Control and Prevention (U.S.)
Allergy and Immunology
Fever
Technology
Sensitivity and Specificity
Serum
Serogroup

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Olsen, S. J., Pruckler, J., Bibb, W., Thanh, N. T. M., Trinh, T. M., Minh, N. T., ... Mintz, E. D. (2004). Evaluation of Rapid Diagnostic Tests for Typhoid Fever. Journal of Clinical Microbiology, 42(5), 1885-1889. https://doi.org/10.1128/JCM.42.5.1885-1889.2004

Evaluation of Rapid Diagnostic Tests for Typhoid Fever. / Olsen, Sonja J.; Pruckler, Jim; Bibb, William; Thanh, Nguyen Thi My; Trinh, Tran My; Minh, Nguyen Thi; Sivapalasingam, Sumathi; Gupta, Amita; Phuong, Phan Thu; Chinh, Nguyen Tran; Chau, Nguyen Vinh; Cam, Phung Dac; Mintz, Eric D.

In: Journal of Clinical Microbiology, Vol. 42, No. 5, 05.2004, p. 1885-1889.

Research output: Contribution to journalArticle

Olsen, SJ, Pruckler, J, Bibb, W, Thanh, NTM, Trinh, TM, Minh, NT, Sivapalasingam, S, Gupta, A, Phuong, PT, Chinh, NT, Chau, NV, Cam, PD & Mintz, ED 2004, 'Evaluation of Rapid Diagnostic Tests for Typhoid Fever', Journal of Clinical Microbiology, vol. 42, no. 5, pp. 1885-1889. https://doi.org/10.1128/JCM.42.5.1885-1889.2004
Olsen SJ, Pruckler J, Bibb W, Thanh NTM, Trinh TM, Minh NT et al. Evaluation of Rapid Diagnostic Tests for Typhoid Fever. Journal of Clinical Microbiology. 2004 May;42(5):1885-1889. https://doi.org/10.1128/JCM.42.5.1885-1889.2004
Olsen, Sonja J. ; Pruckler, Jim ; Bibb, William ; Thanh, Nguyen Thi My ; Trinh, Tran My ; Minh, Nguyen Thi ; Sivapalasingam, Sumathi ; Gupta, Amita ; Phuong, Phan Thu ; Chinh, Nguyen Tran ; Chau, Nguyen Vinh ; Cam, Phung Dac ; Mintz, Eric D. / Evaluation of Rapid Diagnostic Tests for Typhoid Fever. In: Journal of Clinical Microbiology. 2004 ; Vol. 42, No. 5. pp. 1885-1889.
@article{1f27f96b468e443ea34f17fead066d65,
title = "Evaluation of Rapid Diagnostic Tests for Typhoid Fever",
abstract = "Laboratory diagnosis of typhoid fever requires isolation and identification of Salmonella enterica serotype Typhi. In many areas where this disease is endemic, laboratory capability is limited. Recent advances in molecular immunology have led to the identification of sensitive and specific markers for typhoid fever and technology to manufacture practical and inexpensive kits for their rapid detection. We evaluated three commercial kits for serologic diagnosis of typhoid fever. Patients presenting with ≥ 4 days of fever were enrolled at two hospitals in Southern Vietnam. Cases were patients with serotype Typhi isolated from blood samples, and controls were patients with other laboratory-confirmed illnesses. Serotype Typhi isolates were confirmed and tested for antimicrobial susceptibility at the Pasteur Institute in Ho Chi Minh City. The Widal test was run at the hospitals and the Pasteur Institute. Sera were shipped frozen to the Centers for Disease Control and Prevention and tested by using Multi-Test Dip-S-Ticks, TyphiDot, and TUBEX to detect immunoglobulin G (IgG), IgG and IgM, and IgM, respectively. Package insert protocol instructions were followed. We enrolled 59 patients and 21 controls. The sensitivity and specificity findings were as follows: 89 and 53{\%} for Multi-Test Dip-S-Ticks, 79 and 89{\%} for TyphiDot, 78 and 89{\%} for TUBEX, and 64 and 76{\%} for Widal testing in hospitals and 61{\%} and 100{\%} for Widal testing at the Pasteur Institute. For all assays, the sensitivity was highest in the second week of illness. The Widal test was insensitive and displayed interoperator variability. Two rapid kits, TyphiDot and TUBEX, demonstrated promising results.",
author = "Olsen, {Sonja J.} and Jim Pruckler and William Bibb and Thanh, {Nguyen Thi My} and Trinh, {Tran My} and Minh, {Nguyen Thi} and Sumathi Sivapalasingam and Amita Gupta and Phuong, {Phan Thu} and Chinh, {Nguyen Tran} and Chau, {Nguyen Vinh} and Cam, {Phung Dac} and Mintz, {Eric D.}",
year = "2004",
month = "5",
doi = "10.1128/JCM.42.5.1885-1889.2004",
language = "English (US)",
volume = "42",
pages = "1885--1889",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Evaluation of Rapid Diagnostic Tests for Typhoid Fever

AU - Olsen, Sonja J.

AU - Pruckler, Jim

AU - Bibb, William

AU - Thanh, Nguyen Thi My

AU - Trinh, Tran My

AU - Minh, Nguyen Thi

AU - Sivapalasingam, Sumathi

AU - Gupta, Amita

AU - Phuong, Phan Thu

AU - Chinh, Nguyen Tran

AU - Chau, Nguyen Vinh

AU - Cam, Phung Dac

AU - Mintz, Eric D.

PY - 2004/5

Y1 - 2004/5

N2 - Laboratory diagnosis of typhoid fever requires isolation and identification of Salmonella enterica serotype Typhi. In many areas where this disease is endemic, laboratory capability is limited. Recent advances in molecular immunology have led to the identification of sensitive and specific markers for typhoid fever and technology to manufacture practical and inexpensive kits for their rapid detection. We evaluated three commercial kits for serologic diagnosis of typhoid fever. Patients presenting with ≥ 4 days of fever were enrolled at two hospitals in Southern Vietnam. Cases were patients with serotype Typhi isolated from blood samples, and controls were patients with other laboratory-confirmed illnesses. Serotype Typhi isolates were confirmed and tested for antimicrobial susceptibility at the Pasteur Institute in Ho Chi Minh City. The Widal test was run at the hospitals and the Pasteur Institute. Sera were shipped frozen to the Centers for Disease Control and Prevention and tested by using Multi-Test Dip-S-Ticks, TyphiDot, and TUBEX to detect immunoglobulin G (IgG), IgG and IgM, and IgM, respectively. Package insert protocol instructions were followed. We enrolled 59 patients and 21 controls. The sensitivity and specificity findings were as follows: 89 and 53% for Multi-Test Dip-S-Ticks, 79 and 89% for TyphiDot, 78 and 89% for TUBEX, and 64 and 76% for Widal testing in hospitals and 61% and 100% for Widal testing at the Pasteur Institute. For all assays, the sensitivity was highest in the second week of illness. The Widal test was insensitive and displayed interoperator variability. Two rapid kits, TyphiDot and TUBEX, demonstrated promising results.

AB - Laboratory diagnosis of typhoid fever requires isolation and identification of Salmonella enterica serotype Typhi. In many areas where this disease is endemic, laboratory capability is limited. Recent advances in molecular immunology have led to the identification of sensitive and specific markers for typhoid fever and technology to manufacture practical and inexpensive kits for their rapid detection. We evaluated three commercial kits for serologic diagnosis of typhoid fever. Patients presenting with ≥ 4 days of fever were enrolled at two hospitals in Southern Vietnam. Cases were patients with serotype Typhi isolated from blood samples, and controls were patients with other laboratory-confirmed illnesses. Serotype Typhi isolates were confirmed and tested for antimicrobial susceptibility at the Pasteur Institute in Ho Chi Minh City. The Widal test was run at the hospitals and the Pasteur Institute. Sera were shipped frozen to the Centers for Disease Control and Prevention and tested by using Multi-Test Dip-S-Ticks, TyphiDot, and TUBEX to detect immunoglobulin G (IgG), IgG and IgM, and IgM, respectively. Package insert protocol instructions were followed. We enrolled 59 patients and 21 controls. The sensitivity and specificity findings were as follows: 89 and 53% for Multi-Test Dip-S-Ticks, 79 and 89% for TyphiDot, 78 and 89% for TUBEX, and 64 and 76% for Widal testing in hospitals and 61% and 100% for Widal testing at the Pasteur Institute. For all assays, the sensitivity was highest in the second week of illness. The Widal test was insensitive and displayed interoperator variability. Two rapid kits, TyphiDot and TUBEX, demonstrated promising results.

UR - http://www.scopus.com/inward/record.url?scp=2442505820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442505820&partnerID=8YFLogxK

U2 - 10.1128/JCM.42.5.1885-1889.2004

DO - 10.1128/JCM.42.5.1885-1889.2004

M3 - Article

C2 - 15131144

AN - SCOPUS:2442505820

VL - 42

SP - 1885

EP - 1889

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 5

ER -