Evaluation of promoter hypermethylation detection in serum as a diagnostic tool for breast carcinoma in Korean women

Jo Heon Kim, Min Ho Shin, Sun Seog Kweon, Min Ho Park, Jung Han Yoon, Ji Shin Lee, Chan Choi, Mary Jo Fackler, Saraswati Sukumar

Research output: Contribution to journalArticle

Abstract

Objective. We have noted that quantitative multiplex-methylation specific PCR (QM-MSP) analysis of a key panel of genes may be useful as an ancillary tool for ductal carcinoma in situ (DCIS) detection in breast tissue. In this study, we investigated aberrant promoter hypermethylation of four genes as a means to detect epigenetic alterations specific to breast carcinoma in the serum of patients with DCIS and invasive ductal carcinoma (IDC). Methods. Two hundred forty-three serum samples from 89 patients with IDC, 30 patients with DCIS, and 125 age-matched healthy controls were examined. After DNA extraction and sodium bisulfite treatment, QM-MSP was performed for HIN-1, RASSF1A, RAR-β, and Twist. Results. Overall significant differences in methylation levels were observed for HIN-1 (p=0.006), RAR-β (p

Original languageEnglish (US)
Pages (from-to)176-181
Number of pages6
JournalGynecologic Oncology
Volume118
Issue number2
DOIs
StatePublished - Aug 1 2010

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Carcinoma, Intraductal, Noninfiltrating
Methylation
Ductal Carcinoma
Breast Neoplasms
Serum
Polymerase Chain Reaction
Epigenomics
Genes
Breast
DNA
Therapeutics

Keywords

  • Breast
  • Diagnosis
  • Ductal carcinoma in situ
  • Invasive ductal carcinoma
  • Methylation
  • Quantitation
  • Serum

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

Cite this

Evaluation of promoter hypermethylation detection in serum as a diagnostic tool for breast carcinoma in Korean women. / Kim, Jo Heon; Shin, Min Ho; Kweon, Sun Seog; Park, Min Ho; Yoon, Jung Han; Lee, Ji Shin; Choi, Chan; Fackler, Mary Jo; Sukumar, Saraswati.

In: Gynecologic Oncology, Vol. 118, No. 2, 01.08.2010, p. 176-181.

Research output: Contribution to journalArticle

Kim, Jo Heon ; Shin, Min Ho ; Kweon, Sun Seog ; Park, Min Ho ; Yoon, Jung Han ; Lee, Ji Shin ; Choi, Chan ; Fackler, Mary Jo ; Sukumar, Saraswati. / Evaluation of promoter hypermethylation detection in serum as a diagnostic tool for breast carcinoma in Korean women. In: Gynecologic Oncology. 2010 ; Vol. 118, No. 2. pp. 176-181.
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AU - Yoon, Jung Han

AU - Lee, Ji Shin

AU - Choi, Chan

AU - Fackler, Mary Jo

AU - Sukumar, Saraswati

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