TY - JOUR
T1 - Evaluation of myocardial preservation using 31P NMR
AU - Whitman, Glenn J.R.
AU - Roth, Russell A.
AU - Kieval, Robert S.
AU - Harken, Alden H.
PY - 1985/2
Y1 - 1985/2
N2 - The purpose of this study was (1) to monitor myocardial high-energy phosphate content and recovery of left ventricular (LV) contractile function following normothermic graded cardiac ischemia and single-dose hypothermic potassium cardioplegia, and (2) to assess the temporal limits of LV functional recovery during single-dose cardioplegia maintained at 17°C. Rabbit hearts (30) were perfused, equipped with an LV balloon, paced at 240 beats/min, and placed in a nuclear magnetic resonance (NMR) magnet. Hearts underwent either graded, global normothermic ischemia or potassium cardioplegia arrest maintained at 17°C for 1 hr. Myocardial high-energy phosphate level, LV contractility, and temperature were monitored continuously. Phosphocreatine (PCr) fell to 10 ± 2, 2 ± 1, and 0% of control and ATP to 70 ± 3, 19 ± 7, and 0% of control at 10, 40, and 60 min of 37°C ischemia. After 1 hr of reperfusion, regression analysis of final developed pressure (DP) on end ischemic ATP (EIATP) content revealed: DP = 1.02 EIATP + 18 (r = 0.95). Following single-dose cardioplegia, maintained at 17°C, PCr fell to 16 ± 3% of control at 60 min while ATP fell only to 92 ± 5% control. With reperfusion, recovery of DP was 100%. It was concluded that (1) PCr serves as an energy buffer for ATP, (2) EIATP predicts recovery of LV function, (3) single-dose cardioplegia maintained at 17°C provides complete myocardial preservation for up to 60 min.
AB - The purpose of this study was (1) to monitor myocardial high-energy phosphate content and recovery of left ventricular (LV) contractile function following normothermic graded cardiac ischemia and single-dose hypothermic potassium cardioplegia, and (2) to assess the temporal limits of LV functional recovery during single-dose cardioplegia maintained at 17°C. Rabbit hearts (30) were perfused, equipped with an LV balloon, paced at 240 beats/min, and placed in a nuclear magnetic resonance (NMR) magnet. Hearts underwent either graded, global normothermic ischemia or potassium cardioplegia arrest maintained at 17°C for 1 hr. Myocardial high-energy phosphate level, LV contractility, and temperature were monitored continuously. Phosphocreatine (PCr) fell to 10 ± 2, 2 ± 1, and 0% of control and ATP to 70 ± 3, 19 ± 7, and 0% of control at 10, 40, and 60 min of 37°C ischemia. After 1 hr of reperfusion, regression analysis of final developed pressure (DP) on end ischemic ATP (EIATP) content revealed: DP = 1.02 EIATP + 18 (r = 0.95). Following single-dose cardioplegia, maintained at 17°C, PCr fell to 16 ± 3% of control at 60 min while ATP fell only to 92 ± 5% control. With reperfusion, recovery of DP was 100%. It was concluded that (1) PCr serves as an energy buffer for ATP, (2) EIATP predicts recovery of LV function, (3) single-dose cardioplegia maintained at 17°C provides complete myocardial preservation for up to 60 min.
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U2 - 10.1016/0022-4804(85)90022-8
DO - 10.1016/0022-4804(85)90022-8
M3 - Article
C2 - 3968874
AN - SCOPUS:0021924599
SN - 0022-4804
VL - 38
SP - 154
EP - 161
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 2
ER -