Evaluation of multiplexed PCR and liquid-phase array for identification of respiratory fungal pathogens

Daelynn R. Buelow, Zhengming Gu, Thomas J. Walsh, Randall T. Hayden

Research output: Contribution to journalArticlepeer-review

Abstract

Invasive fungal infections are the cause of serious morbidity and high mortality in immunocompromised patients. Early laboratory diagnostic options remain limited; however, rapid detection and accurate identification may improve outcome. Herein, multiplexed PCR followed by liquid-phase array was evaluated for detection and identification of common respiratory fungal pathogens, including Aspergillus fumigatus, Rhizopus microsporus, Scedosporium apiospermum and Fusarium solani. The limit of detection ranged 0.11 ng of DNA, depending on the fungus being tested. Primer cross-reactivity was seen for some fungi: Aspergillus flavus primers detected Aspergillus oryzae; Scedosporium apiospermum primers detected Paecilomyces lilacinus, and Aspergillus terreus primers detected S. apiospermum. PCR followed by liquid-phase array is potentially useful for the identification of clinically relevant fungal pathogens.

Original languageEnglish (US)
Pages (from-to)775-780
Number of pages6
JournalMedical mycology
Volume50
Issue number7
DOIs
StatePublished - Oct 2012

Keywords

  • Fungal detection
  • Luminex
  • Multiplex
  • PCR

ASJC Scopus subject areas

  • Infectious Diseases

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