Evaluation of hybridization conditions for spotted oligonucleotide-based DNA microarrays

Mong Hsun Tsai, Hailing Yan, Xi Chen, G. V.R. Chandramouli, Shuping Zhao, Deborah Coffin, C. Norman Coleman, James B. Mitchell, Eric Y. Chuang

Research output: Contribution to journalReview articlepeer-review

Abstract

We compared different hybridization conditions of oligonucleotide-based DNA microarray to acquire optimized and reliable microarray data. Several parameters were evaluated at different hybridization conditions, including signal-to-background (S:B) ratios, signal dynamic range, usable spots, and reproducibility. Statistical analysis showed that better results were obtained when spotted, presynthesized long oligonucleotide arrays were blocked with succinic anhydride and hybridized at 42°C in the presence of 50% formamide.

Original languageEnglish (US)
Pages (from-to)221-224
Number of pages4
JournalMolecular Biotechnology
Volume29
Issue number3
DOIs
StatePublished - Mar 2005

Keywords

  • Gene expression
  • Hybridization conditions
  • Oligonucleotide microarrays

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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