Evaluation of genetic diversity in the golden apple snail, pomacea canaliculata (Lamarck), from different geographical populations in china by inter simple sequence repeat (ISSR)

Shengzhang Dong, Xuping Shentu, Yinyin Pan, Xu Bai, Xiaoping Yu, Huadi Wang

Research output: Contribution to journalArticlepeer-review

Abstract

The genetic diversity of Pomacea canaliculata, collected from Los Banos (LB) in Philippines and Yuyao (YY), Taizhou (TZ), Fuzhou (FZ), Guangzhou (GZ), Nanning (NN), Kunming (KM) in China, was studied by using the inter simple sequence repeat (ISSR) technique. A total of 498 loci from 140 individuals were amplified with four selected ISSR primers and the percentage of polymorphic loci was 87.35%. At the species level, the Nei's gene diversity (H) was 0.3805 and the Shannon information diversity index (I) was 0.5607. A relatively high level of genetic differentiation among populations was detected based on Nei's gene diversity analysis (Gst = 0.2001) and analysis of molecular variance (AMOVA) analysis (øst = 0.0824), indicating the vast majority of genetic variation that occur within the populations. The limited genetic distance (0.0793) and correlation between genetic distance and geographic distance matrices (r =0.5638, P > 0.5) indicated that, there was no significant geographic heterogeneity among these populations.

Original languageEnglish (US)
Pages (from-to)1777-1783
Number of pages7
JournalAfrican Journal of Biotechnology
Volume10
Issue number10
StatePublished - Mar 2011
Externally publishedYes

Keywords

  • Genetic diversity
  • Geographical populations
  • Inter simple sequence repeat (ISSR)
  • Pomacea canaliculata

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics
  • Agronomy and Crop Science

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