Evaluation of enrichment method for the detection of Vibrio cholerae O1 using a rapid dipstick test in Bangladesh

Christine M. George, Mahamud ur Rashid, David A. Sack, R. Bradley Sack, K. M. Saif-Ur-Rahman, Andrew S. Azman, Shirajum Monira, Sazzadul I. Bhuyian, K. M. Zillur Rahman, M. Toslim Mahmud, Munshi Mustafiz, Munirul Alam

Research output: Contribution to journalArticlepeer-review

Abstract

Background: To evaluate the specificity of the Crystal VC dipstick test for detecting cholera. Methods: We compared direct testing using the Crystal VC dipstick test and testing after enrichment for 6 h in alkaline peptone water (APW) to bacterial culture as the gold standard. Samples positive by dipstick but negative by culture were also tested using PCR. Results: Stool was collected from 125 patients. The overall specificities of the direct testing and testing after 6-h enrichment in APW compared to bacterial culture were 91.8% and 98.4% (P = 0.125), respectively, and the sensitivities were 65.6% and 75.0% (P = 0.07), respectively. Conclusion: The increase in the sensitivity of the Crystal VC kit with the use of the 6-h enrichment step in APW compared to direct testing was marginally significant. The Crystal VC dipstick had a much higher specificity than previously reported (91-98%). Therefore, this method might be a promising screening tool for cholera outbreak surveillance in resource-limited settings where elimination of false-positive results is critical.

Original languageEnglish (US)
Pages (from-to)301-307
Number of pages7
JournalTropical Medicine and International Health
Volume19
Issue number3
DOIs
StatePublished - Mar 2014

Keywords

  • Bangladesh
  • Culture
  • Diagnosis
  • Dipstick test
  • V. cholerae O1

ASJC Scopus subject areas

  • Parasitology
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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