Evaluation of conventional media for detection of colonization factor antigens of enterotoxigenic Escherichia coli

A. R. Ghosh, D. Sen, D. A. Sack, A. T.M.S. Hoque

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Strains of enterotoxigenic Escherichia coli producing either colonization factor antigen (CFA) I or II were tested for expression of CFA when grown on 16 different agar media by using agglutination and coagglutination with monoclonal antibodies, mannose-resistant hemagglutination, and a salt aggregation assay. CFA was detected from the CFA-positive strains when CFA agar was used, and it was also detected when other commercially available media were used, notably nutrient agar. CFA was not detected when other commercial media such as MacConkey agar were used. The use of nutrient agar with monoclonal antibody-based coagglutination reagents offers a potentially simple and rapid method for detecting E. coli which express CFA I or II.

Original languageEnglish (US)
Pages (from-to)2163-2166
Number of pages4
JournalJournal of clinical microbiology
Volume31
Issue number8
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Microbiology (medical)

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