Evaluation of clostridium novyi-NT spores in dogs with naturally occurring tumors

Erika L. Krick, Karin U. Sorenmo, Shelley C. Rankin, Ian Cheong, Barry Kobrin, Katherine Thornton, Kenneth W Kinzler, Bert Vogelstein, Shibin Zhou, Luis A. Diaz

Research output: Contribution to journalArticle

Abstract

Objective-To establish the maximum tolerated dose of Clostridium novyi-NT spores in tumor-bearing dogs and evaluate spore germination within tumors and tumor response. Animals-6 client-owned dogs. Procedures-A standard dose-escalation study was planned, with maximum tolerated dose defined as the highest dose at which 0 or 1 of 6 dogs had dose-limiting toxicoses (DLT). Dogs received 1 dose of C novyi-NT spores IV. Toxicoses were graded and interventions performed according to specific guidelines. Grade 3 or higher toxicosis or any toxicosis combination that substantially affected patient status was considered DLT. Clinical response was measured by use of response evaluation criteria in solid tumors at 28 days. Results-The first 2 dogs had DLT. The dose was decreased. Two of the next 4 dogs had DLT; therefore, dose administration was stopped because the study endpoint had been reached. The most common toxicosis was fever (n = 6 dogs). Two dogs developed abscesses (1 within a nasal carcinoma and 1 splenic abscess) attributable to C novyi-NT infection; both required surgical intervention. Clostridium novyi-NT was cultured from 1 of 6 tumors. Five dogs were available for response assessment (4 had stable disease; 1 had progressive disease). Conclusions and Clinical Relevance-Results indicated that C novyi-NT can germinate within tumors of dogs. Toxicosis, although common and sometimes severe, was manageable with treatment. Further studies in dogs with superficial tumors may allow for continued dose escalation and provide information for use in clinical trials in veterinary and human oncology.

Original languageEnglish (US)
Pages (from-to)112-118
Number of pages7
JournalAmerican Journal of Veterinary Research
Volume73
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Clostridium novyi
Clostridium
Spores
spores
Dogs
poisoning
neoplasms
dogs
dosage
Neoplasms
Maximum Tolerated Dose
abscess
Abscess
Germination
spore germination
Nose
endpoints

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Krick, E. L., Sorenmo, K. U., Rankin, S. C., Cheong, I., Kobrin, B., Thornton, K., ... Diaz, L. A. (2012). Evaluation of clostridium novyi-NT spores in dogs with naturally occurring tumors. American Journal of Veterinary Research, 73(1), 112-118. https://doi.org/10.2460/ajvr.73.1.112

Evaluation of clostridium novyi-NT spores in dogs with naturally occurring tumors. / Krick, Erika L.; Sorenmo, Karin U.; Rankin, Shelley C.; Cheong, Ian; Kobrin, Barry; Thornton, Katherine; Kinzler, Kenneth W; Vogelstein, Bert; Zhou, Shibin; Diaz, Luis A.

In: American Journal of Veterinary Research, Vol. 73, No. 1, 01.2012, p. 112-118.

Research output: Contribution to journalArticle

Krick, Erika L. ; Sorenmo, Karin U. ; Rankin, Shelley C. ; Cheong, Ian ; Kobrin, Barry ; Thornton, Katherine ; Kinzler, Kenneth W ; Vogelstein, Bert ; Zhou, Shibin ; Diaz, Luis A. / Evaluation of clostridium novyi-NT spores in dogs with naturally occurring tumors. In: American Journal of Veterinary Research. 2012 ; Vol. 73, No. 1. pp. 112-118.
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