Evaluation of cell transplant-mediated attenuation of diffuse injury in experimental autoimmune encephalomyelitis using onVDMP CEST MRI

A. M. Thomas; S. Li; C. Chu; I. Shats; J. Xu; P. A. Calabresi; P. C.M. van Zijl; P. Walczak; J. W.M. Bulte

doi:10.1016/j.expneurol.2020.113316

Evaluation of cell transplant-mediated attenuation of diffuse injury in experimental autoimmune encephalomyelitis using onVDMP CEST MRI

A. M. Thomas, S. Li, C. Chu, I. Shats, J. Xu, P. A. Calabresi, P. C.M. van Zijl, P. Walczak, J. W.M. Bulte

Research output: Contribution to journal › Article › peer-review

Abstract

The development and translation of cell therapies have been hindered by an inability to predict and evaluate their efficacy after transplantation. Using an experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis (MS), we studied attenuation of the diffuse injury characteristic of EAE and MS by transplanted glial-restricted precursor cells (GRPs). We assessed the potential of on-resonance variable delay multiple pulse (onVDMP) chemical exchange saturation transfer (CEST) MRI to visualize this attenuation. Allogeneic GRPs transplanted in the motor cortex or lateral ventricles attenuated paralysis in EAE mice and attenuated differences compared to naïve mice in onVDMP CEST signal 5 days after transplantation near the transplantation site. Histological analysis revealed that transplanted GRPs co-localized with attenuated astrogliosis. Hence, diffuse injury-sensitive onVDMP CEST MRI may complement conventional MRI to locate and monitor tissue regions responsive to GRP therapy.

Original language	English (US)
Article number	113316
Journal	Experimental Neurology
Volume	329
DOIs	https://doi.org/10.1016/j.expneurol.2020.113316
State	Published - Jul 2020

Keywords

Bioluminescence imaging
Chemical exchange saturation transfer
Glial-restricted precursor cells
Magnetic resonance imaging
Multiple sclerosis
Transplantation

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

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10.1016/j.expneurol.2020.113316

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title = "Evaluation of cell transplant-mediated attenuation of diffuse injury in experimental autoimmune encephalomyelitis using onVDMP CEST MRI",

abstract = "The development and translation of cell therapies have been hindered by an inability to predict and evaluate their efficacy after transplantation. Using an experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis (MS), we studied attenuation of the diffuse injury characteristic of EAE and MS by transplanted glial-restricted precursor cells (GRPs). We assessed the potential of on-resonance variable delay multiple pulse (onVDMP) chemical exchange saturation transfer (CEST) MRI to visualize this attenuation. Allogeneic GRPs transplanted in the motor cortex or lateral ventricles attenuated paralysis in EAE mice and attenuated differences compared to na{\"i}ve mice in onVDMP CEST signal 5 days after transplantation near the transplantation site. Histological analysis revealed that transplanted GRPs co-localized with attenuated astrogliosis. Hence, diffuse injury-sensitive onVDMP CEST MRI may complement conventional MRI to locate and monitor tissue regions responsive to GRP therapy.",

keywords = "Bioluminescence imaging, Chemical exchange saturation transfer, Glial-restricted precursor cells, Magnetic resonance imaging, Multiple sclerosis, Transplantation",

author = "Thomas, {A. M.} and S. Li and C. Chu and I. Shats and J. Xu and Calabresi, {P. A.} and {van Zijl}, {P. C.M.} and P. Walczak and Bulte, {J. W.M.}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = jul,

doi = "10.1016/j.expneurol.2020.113316",

language = "English (US)",

volume = "329",

journal = "Experimental Neurology",

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AU - Thomas, A. M.

AU - Li, S.

AU - Chu, C.

AU - Shats, I.

AU - Xu, J.

AU - Calabresi, P. A.

AU - van Zijl, P. C.M.

AU - Walczak, P.

AU - Bulte, J. W.M.

PY - 2020/7

Y1 - 2020/7

N2 - The development and translation of cell therapies have been hindered by an inability to predict and evaluate their efficacy after transplantation. Using an experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis (MS), we studied attenuation of the diffuse injury characteristic of EAE and MS by transplanted glial-restricted precursor cells (GRPs). We assessed the potential of on-resonance variable delay multiple pulse (onVDMP) chemical exchange saturation transfer (CEST) MRI to visualize this attenuation. Allogeneic GRPs transplanted in the motor cortex or lateral ventricles attenuated paralysis in EAE mice and attenuated differences compared to naïve mice in onVDMP CEST signal 5 days after transplantation near the transplantation site. Histological analysis revealed that transplanted GRPs co-localized with attenuated astrogliosis. Hence, diffuse injury-sensitive onVDMP CEST MRI may complement conventional MRI to locate and monitor tissue regions responsive to GRP therapy.

AB - The development and translation of cell therapies have been hindered by an inability to predict and evaluate their efficacy after transplantation. Using an experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis (MS), we studied attenuation of the diffuse injury characteristic of EAE and MS by transplanted glial-restricted precursor cells (GRPs). We assessed the potential of on-resonance variable delay multiple pulse (onVDMP) chemical exchange saturation transfer (CEST) MRI to visualize this attenuation. Allogeneic GRPs transplanted in the motor cortex or lateral ventricles attenuated paralysis in EAE mice and attenuated differences compared to naïve mice in onVDMP CEST signal 5 days after transplantation near the transplantation site. Histological analysis revealed that transplanted GRPs co-localized with attenuated astrogliosis. Hence, diffuse injury-sensitive onVDMP CEST MRI may complement conventional MRI to locate and monitor tissue regions responsive to GRP therapy.

KW - Bioluminescence imaging

KW - Chemical exchange saturation transfer

KW - Glial-restricted precursor cells

KW - Magnetic resonance imaging

KW - Multiple sclerosis

KW - Transplantation

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Evaluation of cell transplant-mediated attenuation of diffuse injury in experimental autoimmune encephalomyelitis using onVDMP CEST MRI

Abstract

Keywords

ASJC Scopus subject areas

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