Evaluation of cancer dependence and druggability of PRP4 kinase using cellular, biochemical, and structural approaches

Qiang Gao, Ingrid Mechin, Nayantara Kothari, Zhuyan Guo, Gejing Deng, Kimberly Haas, Jessica McManus, Dietmar Hoffmann, Anlai Wang, Dmitri Wiederschain, Jennifer Rocnik, Werngard Czechtizky, Xin Chen, Larry McLean, Heike Arlt, David Harper, Feng Liu, Tahir Majid, Vinod Patel, Christoph LengauerCarlos Garcia-Echeverria, Bailin Zhang, Hong Cheng, Marion Dorsch, Shih Min A Huang

Research output: Contribution to journalArticle

Abstract

Background: Little is known about the cancer dependence and druggability of PRP4. Results: Significance of PRP4 catalytic activity is demonstrated, novel substrates are identified, and features of kinase domain structure are revealed. Conclusion: PRP4 is required for cancer cell survival, displays substrate specificity, and is amenable to pharmacological inhibition. Significance: Our results indicate that PRP4 is a potential drug target to pursue in cancer.

Original languageEnglish (US)
Pages (from-to)30125-30138
Number of pages14
JournalJournal of Biological Chemistry
Volume288
Issue number42
DOIs
StatePublished - Oct 18 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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  • Cite this

    Gao, Q., Mechin, I., Kothari, N., Guo, Z., Deng, G., Haas, K., McManus, J., Hoffmann, D., Wang, A., Wiederschain, D., Rocnik, J., Czechtizky, W., Chen, X., McLean, L., Arlt, H., Harper, D., Liu, F., Majid, T., Patel, V., ... Huang, S. M. A. (2013). Evaluation of cancer dependence and druggability of PRP4 kinase using cellular, biochemical, and structural approaches. Journal of Biological Chemistry, 288(42), 30125-30138. https://doi.org/10.1074/jbc.M113.473348