Evaluation of apigenin using in vitro cytochalasin blocked micronucleus assay

Sanjeev Noel, Madhu Kasinathan, Srikanta Kumar Rath

Research output: Contribution to journalArticlepeer-review

Abstract

High doses of flavonoids are reported to be clastogenic in contrast to their potential to reduce oxidative DNA damage, retard growth of leukemia cells, obstruct cell signal transduction and induce cellular differentiation in cancers. In the present study, we evaluated apigenin, a plant-derived flavonoid in doses of 10, 33, and 100 μM per 5 ml culture using cytochalasin-B blocked micronucleus (CBMN) assay in peripheral human lymphocytes. Apigenin was found to induce micronuclei in a dose dependent manner indicating potential genotoxic hazard in humans. Hence, flavonoids may act as mutagen, pro-oxidant or as inhibitor of key enzymes to produce clastogenic effects depending upon the levels consumed as well as the physiological parameters.

Original languageEnglish (US)
Pages (from-to)1168-1172
Number of pages5
JournalToxicology in Vitro
Volume20
Issue number7
DOIs
StatePublished - Oct 2006
Externally publishedYes

Keywords

  • Apigenin
  • CBMN assay
  • Cytochalasin-B
  • Genotoxicity
  • Human peripheral lymphocytes
  • Micronucleus

ASJC Scopus subject areas

  • Toxicology

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