Evaluation of apigenin using in vitro cytochalasin blocked micronucleus assay

Sanjeev Noel; Madhu Kasinathan; Srikanta Kumar Rath

doi:10.1016/j.tiv.2006.03.007

Evaluation of apigenin using in vitro cytochalasin blocked micronucleus assay

Sanjeev Noel, Madhu Kasinathan, Srikanta Kumar Rath

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

High doses of flavonoids are reported to be clastogenic in contrast to their potential to reduce oxidative DNA damage, retard growth of leukemia cells, obstruct cell signal transduction and induce cellular differentiation in cancers. In the present study, we evaluated apigenin, a plant-derived flavonoid in doses of 10, 33, and 100 μM per 5 ml culture using cytochalasin-B blocked micronucleus (CBMN) assay in peripheral human lymphocytes. Apigenin was found to induce micronuclei in a dose dependent manner indicating potential genotoxic hazard in humans. Hence, flavonoids may act as mutagen, pro-oxidant or as inhibitor of key enzymes to produce clastogenic effects depending upon the levels consumed as well as the physiological parameters.

Original language	English (US)
Pages (from-to)	1168-1172
Number of pages	5
Journal	Toxicology in Vitro
Volume	20
Issue number	7
DOIs	https://doi.org/10.1016/j.tiv.2006.03.007
State	Published - Oct 2006
Externally published	Yes

Keywords

Apigenin
CBMN assay
Cytochalasin-B
Genotoxicity
Human peripheral lymphocytes
Micronucleus

ASJC Scopus subject areas

Toxicology

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10.1016/j.tiv.2006.03.007

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title = "Evaluation of apigenin using in vitro cytochalasin blocked micronucleus assay",

abstract = "High doses of flavonoids are reported to be clastogenic in contrast to their potential to reduce oxidative DNA damage, retard growth of leukemia cells, obstruct cell signal transduction and induce cellular differentiation in cancers. In the present study, we evaluated apigenin, a plant-derived flavonoid in doses of 10, 33, and 100 μM per 5 ml culture using cytochalasin-B blocked micronucleus (CBMN) assay in peripheral human lymphocytes. Apigenin was found to induce micronuclei in a dose dependent manner indicating potential genotoxic hazard in humans. Hence, flavonoids may act as mutagen, pro-oxidant or as inhibitor of key enzymes to produce clastogenic effects depending upon the levels consumed as well as the physiological parameters.",

keywords = "Apigenin, CBMN assay, Cytochalasin-B, Genotoxicity, Human peripheral lymphocytes, Micronucleus",

author = "Sanjeev Noel and Madhu Kasinathan and Rath, {Srikanta Kumar}",

note = "Funding Information: Authors are grateful to Dr. C.M. Gupta, Director CDRI and Dr. Sudhir Srivastava, Head Toxicology Division, Central Drug Research Institute, for their interest in this study. We are thankful to Mr. Ashok Singh, Mr. Amit Kumar Mitra, Mr. Ravi Kumar Lella and Dr. Neetu Singh for technical support and critically reviewing the manuscript. One of the authors (S.N.) is a recipient of SRFship from CSIR. This study was funded by CSIR network project CMM0018. This paper bears the publication no. 6874 of CDRI.",

year = "2006",

month = oct,

doi = "10.1016/j.tiv.2006.03.007",

language = "English (US)",

volume = "20",

pages = "1168--1172",

journal = "Toxicology in Vitro",

issn = "0887-2333",

publisher = "Elsevier Limited",

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T1 - Evaluation of apigenin using in vitro cytochalasin blocked micronucleus assay

AU - Noel, Sanjeev

AU - Kasinathan, Madhu

AU - Rath, Srikanta Kumar

N1 - Funding Information: Authors are grateful to Dr. C.M. Gupta, Director CDRI and Dr. Sudhir Srivastava, Head Toxicology Division, Central Drug Research Institute, for their interest in this study. We are thankful to Mr. Ashok Singh, Mr. Amit Kumar Mitra, Mr. Ravi Kumar Lella and Dr. Neetu Singh for technical support and critically reviewing the manuscript. One of the authors (S.N.) is a recipient of SRFship from CSIR. This study was funded by CSIR network project CMM0018. This paper bears the publication no. 6874 of CDRI.

PY - 2006/10

Y1 - 2006/10

N2 - High doses of flavonoids are reported to be clastogenic in contrast to their potential to reduce oxidative DNA damage, retard growth of leukemia cells, obstruct cell signal transduction and induce cellular differentiation in cancers. In the present study, we evaluated apigenin, a plant-derived flavonoid in doses of 10, 33, and 100 μM per 5 ml culture using cytochalasin-B blocked micronucleus (CBMN) assay in peripheral human lymphocytes. Apigenin was found to induce micronuclei in a dose dependent manner indicating potential genotoxic hazard in humans. Hence, flavonoids may act as mutagen, pro-oxidant or as inhibitor of key enzymes to produce clastogenic effects depending upon the levels consumed as well as the physiological parameters.

AB - High doses of flavonoids are reported to be clastogenic in contrast to their potential to reduce oxidative DNA damage, retard growth of leukemia cells, obstruct cell signal transduction and induce cellular differentiation in cancers. In the present study, we evaluated apigenin, a plant-derived flavonoid in doses of 10, 33, and 100 μM per 5 ml culture using cytochalasin-B blocked micronucleus (CBMN) assay in peripheral human lymphocytes. Apigenin was found to induce micronuclei in a dose dependent manner indicating potential genotoxic hazard in humans. Hence, flavonoids may act as mutagen, pro-oxidant or as inhibitor of key enzymes to produce clastogenic effects depending upon the levels consumed as well as the physiological parameters.

KW - Apigenin

KW - CBMN assay

KW - Cytochalasin-B

KW - Genotoxicity

KW - Human peripheral lymphocytes

KW - Micronucleus

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Evaluation of apigenin using in vitro cytochalasin blocked micronucleus assay

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