Evaluation of anti-apoptotic activity of different dietary antioxidants in renal cell carcinoma against hydrogen peroxide

K. Garg Neeraj, Mangal Sharad, Sahu Tejram, Mehta Abhinav, P. Vyas Suresh, K. Tyagi Rajeev

Research output: Contribution to journalArticle

Abstract

Objective: To evaluate the anti-apoptotic and radical scavenging activities of dietary phenolics, namely ascorbic acid, α -tocopherol acetate, citric acid, salicylic acid, and estimate H2O2-induced apoptosis in renal cell carcinoma cells. Methods: The intracellular antioxidant potency of antioxidants was investigated. H2O2-induced apoptosis in RCC-26 was assayed with the following parameters: cell viability (% apoptosis), nucleosomal damage and DNA fragmentation, bcl-2 levels and flow cytometery analysis (ROS production evaluation). Results: The anticancer properties of antioxidants such as ascorbic acid, α - tocopherol acetate, citric acid, salicylic acid with perdurable responses were investigated. It was observed that these antioxidants had protective effect (anti-apoptotic activity) against hydrogen peroxide (H2O2) in renal cell carcinoma (RCC-26) cell line. Conclusions: This study reveals and proves the anticancer properties. However, in cancer cell lines anti-apoptotic activity can indirectly reflect the cancer promoter activity through radicals scavenging, and significantly protect nucleus and bcl-2.

Original languageEnglish (US)
Pages (from-to)57-63
Number of pages7
JournalAsian Pacific Journal of Tropical Biomedicine
Volume1
Issue number1
DOIs
StatePublished - Feb 2011

Keywords

  • Anticancer
  • Antioxidants
  • Apoptosis
  • Hydrogen peroxide
  • RCC-26
  • ROS

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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