Abstract
Objective: To evaluate the anti-apoptotic and radical scavenging activities of dietary phenolics, namely ascorbic acid, α -tocopherol acetate, citric acid, salicylic acid, and estimate H2O2-induced apoptosis in renal cell carcinoma cells. Methods: The intracellular antioxidant potency of antioxidants was investigated. H2O2-induced apoptosis in RCC-26 was assayed with the following parameters: cell viability (% apoptosis), nucleosomal damage and DNA fragmentation, bcl-2 levels and flow cytometery analysis (ROS production evaluation). Results: The anticancer properties of antioxidants such as ascorbic acid, α - tocopherol acetate, citric acid, salicylic acid with perdurable responses were investigated. It was observed that these antioxidants had protective effect (anti-apoptotic activity) against hydrogen peroxide (H2O2) in renal cell carcinoma (RCC-26) cell line. Conclusions: This study reveals and proves the anticancer properties. However, in cancer cell lines anti-apoptotic activity can indirectly reflect the cancer promoter activity through radicals scavenging, and significantly protect nucleus and bcl-2.
Original language | English (US) |
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Pages (from-to) | 57-63 |
Number of pages | 7 |
Journal | Asian Pacific Journal of Tropical Biomedicine |
Volume | 1 |
Issue number | 1 |
DOIs | |
State | Published - Feb 2011 |
Externally published | Yes |
Keywords
- Anticancer
- Antioxidants
- Apoptosis
- Hydrogen peroxide
- RCC-26
- ROS
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology (miscellaneous)