Evaluation of an autoregulatory tetracycline regulated system

Gary L Gallia, Kamel Khalili

Research output: Contribution to journalArticle

Abstract

Tetracycline controlled gene expression systems have become powerful tools in the analysis of gene function in mammalian cell culture as well as transgenic animals and plants. The original description of a tetracycline-regulated gene expression system is based on two plasmids, one of which constitutively expresses a tetracycline-controlled transactivator protein (tTA), a fusion protein between the tetracycline repressor of E. coli and the transcriptional activation domain of the VP16 protein of herpes simplex virus. The second plasmid contains the gene to be regulated by tTA under the control of an inducible promoter which consists of seven copies of the tetracycline resistance operator (tetO). Since this original description, many modifications have been described. In this report, we evaluate an autoregulatory tetracycline controlled system, in which the tTA is itself under the control of the tetO. We demonstrate that this autoregulatory tetracycline system produces adverse effects including cellular morphologic changes, growth rate attenuation and alterations in cell cycle distribution.

Original languageEnglish (US)
Pages (from-to)1879-1884
Number of pages6
JournalOncogene
Volume16
Issue number14
StatePublished - Apr 9 1998
Externally publishedYes

Fingerprint

Tetracycline
Trans-Activators
Tetracycline Resistance
Herpes Simplex Virus Protein Vmw65
Proteins
Plasmids
Gene Expression
Genetically Modified Animals
Genetically Modified Plants
Transcriptional Activation
Genes
Cell Cycle
Cell Culture Techniques
Escherichia coli
Growth

Keywords

  • Gene expression regulation
  • Inducible promoter
  • Tetracycline
  • Transactivator

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Evaluation of an autoregulatory tetracycline regulated system. / Gallia, Gary L; Khalili, Kamel.

In: Oncogene, Vol. 16, No. 14, 09.04.1998, p. 1879-1884.

Research output: Contribution to journalArticle

Gallia, GL & Khalili, K 1998, 'Evaluation of an autoregulatory tetracycline regulated system', Oncogene, vol. 16, no. 14, pp. 1879-1884.
Gallia, Gary L ; Khalili, Kamel. / Evaluation of an autoregulatory tetracycline regulated system. In: Oncogene. 1998 ; Vol. 16, No. 14. pp. 1879-1884.
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