Evaluation of an autoregulatory tetracycline regulated system

Gary L. Gallia, Kamel Khalili

Research output: Contribution to journalArticlepeer-review

Abstract

Tetracycline controlled gene expression systems have become powerful tools in the analysis of gene function in mammalian cell culture as well as transgenic animals and plants. The original description of a tetracycline-regulated gene expression system is based on two plasmids, one of which constitutively expresses a tetracycline-controlled transactivator protein (tTA), a fusion protein between the tetracycline repressor of E. coli and the transcriptional activation domain of the VP16 protein of herpes simplex virus. The second plasmid contains the gene to be regulated by tTA under the control of an inducible promoter which consists of seven copies of the tetracycline resistance operator (tetO). Since this original description, many modifications have been described. In this report, we evaluate an autoregulatory tetracycline controlled system, in which the tTA is itself under the control of the tetO. We demonstrate that this autoregulatory tetracycline system produces adverse effects including cellular morphologic changes, growth rate attenuation and alterations in cell cycle distribution.

Original languageEnglish (US)
Pages (from-to)1879-1884
Number of pages6
JournalOncogene
Volume16
Issue number14
DOIs
StatePublished - Apr 9 1998

Keywords

  • Gene expression regulation
  • Inducible promoter
  • Tetracycline
  • Transactivator

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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