TY - JOUR
T1 - Evaluation of alkaline phosphatase-labelled ipaH probe for diagnosis of Shigella infections
AU - Oberhelman, R. A.
AU - Kopecko, D. J.
AU - Venkatesan, M. M.
AU - Salazar-Lindo, E.
AU - Gotuzzo, E.
AU - Yi, A.
AU - Chea-Woo, E.
AU - Ruiz, R.
AU - Fernandez-Prada, C.
AU - Leon-Barua, R.
AU - Sack, R. B.
PY - 1993
Y1 - 1993
N2 - The presence of many enteropathogens which are not easily detectable by routine stool culture has led to the development of alternative diagnostic methods. One of these techniques, nucleic acid probe hybridization, has been used to identify Shigella spp. and enteroinvasive Escherichia coli (EIEC) in stool specimens through the detection of genetic material encoded by a specific large ≃200-kbp virulence-related plasmid. In the present study, an alkaline phosphatase-labelled oligonucleotide probe developed to detect the gene for ipaH, a repetitive genetic sequence thought to be present on both the virulence-related plasmid and the chromosomes of all strains of Shigella and EIEC, was tested in a developing-country setting through a prospective clinical trial. In a group of 219 Peruvian adults and children with acute gastroenteritis, the ipaH probe detected 85% of cases of shigellosis and demonstrated a specificity of 95% when compared with simultaneous detection by several stool culture techniques. Additionally, three cases of EIEC infection which could not be diagnosed by culture methods alone were detected with the ipaH probe and were confirmed by plasmid analysis and Sereny testing. These preliminary results suggest that, with further research, the ipaH probe should prove to be a useful and rapid adjunct in the diagnosis of acute gastroenteritis in developing countries.
AB - The presence of many enteropathogens which are not easily detectable by routine stool culture has led to the development of alternative diagnostic methods. One of these techniques, nucleic acid probe hybridization, has been used to identify Shigella spp. and enteroinvasive Escherichia coli (EIEC) in stool specimens through the detection of genetic material encoded by a specific large ≃200-kbp virulence-related plasmid. In the present study, an alkaline phosphatase-labelled oligonucleotide probe developed to detect the gene for ipaH, a repetitive genetic sequence thought to be present on both the virulence-related plasmid and the chromosomes of all strains of Shigella and EIEC, was tested in a developing-country setting through a prospective clinical trial. In a group of 219 Peruvian adults and children with acute gastroenteritis, the ipaH probe detected 85% of cases of shigellosis and demonstrated a specificity of 95% when compared with simultaneous detection by several stool culture techniques. Additionally, three cases of EIEC infection which could not be diagnosed by culture methods alone were detected with the ipaH probe and were confirmed by plasmid analysis and Sereny testing. These preliminary results suggest that, with further research, the ipaH probe should prove to be a useful and rapid adjunct in the diagnosis of acute gastroenteritis in developing countries.
UR - http://www.scopus.com/inward/record.url?scp=0027257451&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027257451&partnerID=8YFLogxK
U2 - 10.1128/jcm.31.8.2101-2104.1993
DO - 10.1128/jcm.31.8.2101-2104.1993
M3 - Article
C2 - 8370736
AN - SCOPUS:0027257451
SN - 0095-1137
VL - 31
SP - 2101
EP - 2104
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 8
ER -