Evaluation of A/Alaska/6/77 (H3N2) cold-adapted recombinant viruses derived from A/Ann Arbor/6/60 cold-adapted donor virus in adult seronegative volunteers

Brian R. Murphy; Robert M. Chanock; Mary Lou Clements; William C. Anthony; Alfred J. Sear; Luis A. Cisneros; Margaret B. Rennels; E. Huxley Miller; Robert E. Black; Myron M. Levine; Robert F. Betts; R. Gordon Douglas; Hunnien F. Maassab; Nancy J. Cox; Alan P. Kendal

doi:10.1128/iai.32.2.693-697.1981

Evaluation of A/Alaska/6/77 (H3N2) cold-adapted recombinant viruses derived from A/Ann Arbor/6/60 cold-adapted donor virus in adult seronegative volunteers

Brian R. Murphy, Robert M. Chanock, Mary Lou Clements, William C. Anthony, Alfred J. Sear, Luis A. Cisneros, Margaret B. Rennels, E. Huxley Miller, Robert E. Black, Myron M. Levine, Robert F. Betts, R. Gordon Douglas, Hunnien F. Maassab, Nancy J. Cox, Alan P. Kendal

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33 Scopus citations

Abstract

The influenza A/Ann Arbor/6/60 (H2N2) cold-adapted (ca) virus was evaluated as a donor of attenuating genes to new variants of influenza A virus. This ca donor virus was mated with the A/Alaska/6/77 (H3N2) wild-type virus, and three A/Alaska/6/77 (H3N2) ca recombinant viruses were produced. The parental origin of the genes in the three ca recombinants had been determined previously, and their virulence for adult seronegative volunteers was assessed in the present study to identify the genes present in the ca donor virus that confer attenuation. Each of the recombinants received the hemagglutinin and neuraminidase genes from the A/Alaska/6/77 (H3N2) wild-type parent. One ca recombinant (CR-29) received all six transferable genes from the ca parent and was found to be satisfactorily attenuated in the volunteers. The two other ca recombinants received five of the six transferable genes with a wild-type gene at the M or NS locus. The pattern of infection in humans with these latter two ca recombinants was similar to the CR-29 ca recombinant. These findings demonstrate that inheritance of a gene in ca recombinants at the M or NS locus segregates independently of attenuation and suggest that the M and NS genes present in the ca donor virus are not the major determinants of attenuation conferred by this virus.

Original language	English (US)
Pages (from-to)	693-697
Number of pages	5
Journal	Unknown Journal
Volume	32
Issue number	2
DOIs	https://doi.org/10.1128/iai.32.2.693-697.1981
State	Published - 1981
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

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10.1128/iai.32.2.693-697.1981

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Murphy, B. R., Chanock, R. M., Lou Clements, M., Anthony, W. C., Sear, A. J., Cisneros, L. A., Rennels, M. B., Huxley Miller, E., Black, R. E., Levine, M. M., Betts, R. F., Gordon Douglas, R., Maassab, H. F., Cox, N. J., & Kendal, A. P. (1981). Evaluation of A/Alaska/6/77 (H3N2) cold-adapted recombinant viruses derived from A/Ann Arbor/6/60 cold-adapted donor virus in adult seronegative volunteers. Unknown Journal, 32(2), 693-697. https://doi.org/10.1128/iai.32.2.693-697.1981

Murphy, BR, Chanock, RM, Lou Clements, M, Anthony, WC, Sear, AJ, Cisneros, LA, Rennels, MB, Huxley Miller, E, Black, RE, Levine, MM, Betts, RF, Gordon Douglas, R, Maassab, HF, Cox, NJ & Kendal, AP 1981, 'Evaluation of A/Alaska/6/77 (H3N2) cold-adapted recombinant viruses derived from A/Ann Arbor/6/60 cold-adapted donor virus in adult seronegative volunteers', Unknown Journal, vol. 32, no. 2, pp. 693-697. https://doi.org/10.1128/iai.32.2.693-697.1981

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title = "Evaluation of A/Alaska/6/77 (H3N2) cold-adapted recombinant viruses derived from A/Ann Arbor/6/60 cold-adapted donor virus in adult seronegative volunteers",

abstract = "The influenza A/Ann Arbor/6/60 (H2N2) cold-adapted (ca) virus was evaluated as a donor of attenuating genes to new variants of influenza A virus. This ca donor virus was mated with the A/Alaska/6/77 (H3N2) wild-type virus, and three A/Alaska/6/77 (H3N2) ca recombinant viruses were produced. The parental origin of the genes in the three ca recombinants had been determined previously, and their virulence for adult seronegative volunteers was assessed in the present study to identify the genes present in the ca donor virus that confer attenuation. Each of the recombinants received the hemagglutinin and neuraminidase genes from the A/Alaska/6/77 (H3N2) wild-type parent. One ca recombinant (CR-29) received all six transferable genes from the ca parent and was found to be satisfactorily attenuated in the volunteers. The two other ca recombinants received five of the six transferable genes with a wild-type gene at the M or NS locus. The pattern of infection in humans with these latter two ca recombinants was similar to the CR-29 ca recombinant. These findings demonstrate that inheritance of a gene in ca recombinants at the M or NS locus segregates independently of attenuation and suggest that the M and NS genes present in the ca donor virus are not the major determinants of attenuation conferred by this virus.",

author = "Murphy, {Brian R.} and Chanock, {Robert M.} and {Lou Clements}, Mary and Anthony, {William C.} and Sear, {Alfred J.} and Cisneros, {Luis A.} and Rennels, {Margaret B.} and {Huxley Miller}, E. and Black, {Robert E.} and Levine, {Myron M.} and Betts, {Robert F.} and {Gordon Douglas}, R. and Maassab, {Hunnien F.} and Cox, {Nancy J.} and Kendal, {Alan P.}",

year = "1981",

doi = "10.1128/iai.32.2.693-697.1981",

language = "English (US)",

volume = "32",

pages = "693--697",

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T1 - Evaluation of A/Alaska/6/77 (H3N2) cold-adapted recombinant viruses derived from A/Ann Arbor/6/60 cold-adapted donor virus in adult seronegative volunteers

AU - Murphy, Brian R.

AU - Chanock, Robert M.

AU - Lou Clements, Mary

AU - Anthony, William C.

AU - Sear, Alfred J.

AU - Cisneros, Luis A.

AU - Rennels, Margaret B.

AU - Huxley Miller, E.

AU - Black, Robert E.

AU - Levine, Myron M.

AU - Betts, Robert F.

AU - Gordon Douglas, R.

AU - Maassab, Hunnien F.

AU - Cox, Nancy J.

AU - Kendal, Alan P.

PY - 1981

Y1 - 1981

N2 - The influenza A/Ann Arbor/6/60 (H2N2) cold-adapted (ca) virus was evaluated as a donor of attenuating genes to new variants of influenza A virus. This ca donor virus was mated with the A/Alaska/6/77 (H3N2) wild-type virus, and three A/Alaska/6/77 (H3N2) ca recombinant viruses were produced. The parental origin of the genes in the three ca recombinants had been determined previously, and their virulence for adult seronegative volunteers was assessed in the present study to identify the genes present in the ca donor virus that confer attenuation. Each of the recombinants received the hemagglutinin and neuraminidase genes from the A/Alaska/6/77 (H3N2) wild-type parent. One ca recombinant (CR-29) received all six transferable genes from the ca parent and was found to be satisfactorily attenuated in the volunteers. The two other ca recombinants received five of the six transferable genes with a wild-type gene at the M or NS locus. The pattern of infection in humans with these latter two ca recombinants was similar to the CR-29 ca recombinant. These findings demonstrate that inheritance of a gene in ca recombinants at the M or NS locus segregates independently of attenuation and suggest that the M and NS genes present in the ca donor virus are not the major determinants of attenuation conferred by this virus.

AB - The influenza A/Ann Arbor/6/60 (H2N2) cold-adapted (ca) virus was evaluated as a donor of attenuating genes to new variants of influenza A virus. This ca donor virus was mated with the A/Alaska/6/77 (H3N2) wild-type virus, and three A/Alaska/6/77 (H3N2) ca recombinant viruses were produced. The parental origin of the genes in the three ca recombinants had been determined previously, and their virulence for adult seronegative volunteers was assessed in the present study to identify the genes present in the ca donor virus that confer attenuation. Each of the recombinants received the hemagglutinin and neuraminidase genes from the A/Alaska/6/77 (H3N2) wild-type parent. One ca recombinant (CR-29) received all six transferable genes from the ca parent and was found to be satisfactorily attenuated in the volunteers. The two other ca recombinants received five of the six transferable genes with a wild-type gene at the M or NS locus. The pattern of infection in humans with these latter two ca recombinants was similar to the CR-29 ca recombinant. These findings demonstrate that inheritance of a gene in ca recombinants at the M or NS locus segregates independently of attenuation and suggest that the M and NS genes present in the ca donor virus are not the major determinants of attenuation conferred by this virus.

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Evaluation of A/Alaska/6/77 (H3N2) cold-adapted recombinant viruses derived from A/Ann Arbor/6/60 cold-adapted donor virus in adult seronegative volunteers

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