Evaluation of a whole-blood interferon-γ release assay for the detection of Mycobacterium tuberculosis infection in 2 study populations

Bahrie Bellete, Jacqueline Coberly, Grace Link Barnes, Chiew Ko, Richard E Chaisson, George W. Comstock, William Ramses Bishai

Research output: Contribution to journalArticle

Abstract

A whole-blood interferon-γ release assay (IGRA) is being evaluated for its potential to replace the tuberculin skin test (TST) for detecting Mycobacteriurn tuberculosis infection. To test the assay in a population in which tuberculosis is highly endemic and in another population that is representative of an urban United States population, 253 volunteers from Ethiopia and 175 volunteers from Baltimore were studied for responsiveness on IGRA compared with a simultaneously performed TST. The agreement between the 2 tests, beyond that due to chance, was 68% among subjects from Baltimore and only 35% among those from Ethiopia. IGRA had a sensitivity of 71%, compared with 95% sensitivity for the TST, among 21 subjects who had undergone treatment for culture-confirmed tuberculosis. The specificity was 85% for IGRA and 96% for TST among 52 subjects with no known history of exposure to tuberculosis. In its current form, with purified protein derivative used as the stimulation antigen, the IGRA was found to perform poorly in comparison to the TST in diagnosing M. tuberculosis infection.

Original languageEnglish (US)
Pages (from-to)1449-1456
Number of pages8
JournalClinical Infectious Diseases
Volume34
Issue number11
DOIs
StatePublished - Jun 1 2002

Fingerprint

Tuberculin Test
Mycobacterium Infections
Skin Tests
Mycobacterium tuberculosis
Interferons
Tuberculosis
Baltimore
Ethiopia
Population
Volunteers
Antigens
Infection
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Evaluation of a whole-blood interferon-γ release assay for the detection of Mycobacterium tuberculosis infection in 2 study populations. / Bellete, Bahrie; Coberly, Jacqueline; Link Barnes, Grace; Ko, Chiew; Chaisson, Richard E; Comstock, George W.; Bishai, William Ramses.

In: Clinical Infectious Diseases, Vol. 34, No. 11, 01.06.2002, p. 1449-1456.

Research output: Contribution to journalArticle

@article{a6104a7fa2ca4bacbc9d41a13d9c57e0,
title = "Evaluation of a whole-blood interferon-γ release assay for the detection of Mycobacterium tuberculosis infection in 2 study populations",
abstract = "A whole-blood interferon-γ release assay (IGRA) is being evaluated for its potential to replace the tuberculin skin test (TST) for detecting Mycobacteriurn tuberculosis infection. To test the assay in a population in which tuberculosis is highly endemic and in another population that is representative of an urban United States population, 253 volunteers from Ethiopia and 175 volunteers from Baltimore were studied for responsiveness on IGRA compared with a simultaneously performed TST. The agreement between the 2 tests, beyond that due to chance, was 68{\%} among subjects from Baltimore and only 35{\%} among those from Ethiopia. IGRA had a sensitivity of 71{\%}, compared with 95{\%} sensitivity for the TST, among 21 subjects who had undergone treatment for culture-confirmed tuberculosis. The specificity was 85{\%} for IGRA and 96{\%} for TST among 52 subjects with no known history of exposure to tuberculosis. In its current form, with purified protein derivative used as the stimulation antigen, the IGRA was found to perform poorly in comparison to the TST in diagnosing M. tuberculosis infection.",
author = "Bahrie Bellete and Jacqueline Coberly and {Link Barnes}, Grace and Chiew Ko and Chaisson, {Richard E} and Comstock, {George W.} and Bishai, {William Ramses}",
year = "2002",
month = "6",
day = "1",
doi = "10.1086/340397",
language = "English (US)",
volume = "34",
pages = "1449--1456",
journal = "Clinical Infectious Diseases",
issn = "1058-4838",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - Evaluation of a whole-blood interferon-γ release assay for the detection of Mycobacterium tuberculosis infection in 2 study populations

AU - Bellete, Bahrie

AU - Coberly, Jacqueline

AU - Link Barnes, Grace

AU - Ko, Chiew

AU - Chaisson, Richard E

AU - Comstock, George W.

AU - Bishai, William Ramses

PY - 2002/6/1

Y1 - 2002/6/1

N2 - A whole-blood interferon-γ release assay (IGRA) is being evaluated for its potential to replace the tuberculin skin test (TST) for detecting Mycobacteriurn tuberculosis infection. To test the assay in a population in which tuberculosis is highly endemic and in another population that is representative of an urban United States population, 253 volunteers from Ethiopia and 175 volunteers from Baltimore were studied for responsiveness on IGRA compared with a simultaneously performed TST. The agreement between the 2 tests, beyond that due to chance, was 68% among subjects from Baltimore and only 35% among those from Ethiopia. IGRA had a sensitivity of 71%, compared with 95% sensitivity for the TST, among 21 subjects who had undergone treatment for culture-confirmed tuberculosis. The specificity was 85% for IGRA and 96% for TST among 52 subjects with no known history of exposure to tuberculosis. In its current form, with purified protein derivative used as the stimulation antigen, the IGRA was found to perform poorly in comparison to the TST in diagnosing M. tuberculosis infection.

AB - A whole-blood interferon-γ release assay (IGRA) is being evaluated for its potential to replace the tuberculin skin test (TST) for detecting Mycobacteriurn tuberculosis infection. To test the assay in a population in which tuberculosis is highly endemic and in another population that is representative of an urban United States population, 253 volunteers from Ethiopia and 175 volunteers from Baltimore were studied for responsiveness on IGRA compared with a simultaneously performed TST. The agreement between the 2 tests, beyond that due to chance, was 68% among subjects from Baltimore and only 35% among those from Ethiopia. IGRA had a sensitivity of 71%, compared with 95% sensitivity for the TST, among 21 subjects who had undergone treatment for culture-confirmed tuberculosis. The specificity was 85% for IGRA and 96% for TST among 52 subjects with no known history of exposure to tuberculosis. In its current form, with purified protein derivative used as the stimulation antigen, the IGRA was found to perform poorly in comparison to the TST in diagnosing M. tuberculosis infection.

UR - http://www.scopus.com/inward/record.url?scp=0036604141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036604141&partnerID=8YFLogxK

U2 - 10.1086/340397

DO - 10.1086/340397

M3 - Article

VL - 34

SP - 1449

EP - 1456

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

SN - 1058-4838

IS - 11

ER -