Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection

Rosario Vivek, Syed Fazil Ahamed, Shalini Kotabagi, Anmol Chandele, Ira Khanna, Navin Khanna, Kaustuv Nayak, Mary Dias, Murali Krishna Kaja, Anita Shet

Research output: Contribution to journalArticle

Abstract

The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotypes in southern India. The dengue-positive panel consisted of 179 dengue real-time polymerase chain reaction (RT-PCR) positive samples from symptomatic children. The dengue-negative panel consisted of 32 samples from dengue-negative febrile children and asymptomatic individuals that were negative for dengue RT-PCR/NS1 enzyme-linked immunosorbent assay/IgM/IgG. NS1/IgM RDT sensitivity was 89.4% and specificity was 93.8%. The NS1/IgM RDT showed high sensitivity throughout the acute phase of illness, in primary and secondary infections, in different severity groups, and detected all 4 dengue serotypes, including coinfections. This NS1/IgM RDT is a useful point-of-care assay for rapid and reliable diagnosis of acute dengue and an excellent surveillance tool in our battle against dengue.

Original languageEnglish (US)
Pages (from-to)229-234
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume87
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

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Keywords

  • Dengue
  • Diagnostics
  • Evaluation
  • NS1 protein
  • Rapid diagnostic test

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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