Evaluation of a multiplex PCR assay to rapidly detect Enterobacteriaceae with a broad range of β-lactamases directly from perianal swabs

Kalyan D. Chavda, Michael J. Satlin, Liang Chen, Claudia Manca, Stephen G. Jenkins, Thomas J. Walsh, Barry N. Kreiswirth

Research output: Contribution to journalArticlepeer-review

Abstract

We developed and evaluated multiplexed molecular beacon probes in a real-time PCR assay to identify prominent extended-spectrum-β-lactamase, plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemase genes directly from perianal swab specimens within 6 h. We evaluated this assay on 158 perianal swabs collected from hematopoietic stem cell transplant recipients and found that this assay was highly sensitive and specific for detection of CTX-M-, pAmpC-, and KPC-producing Enterobacteriaceae compared to culture on chromogenic agar.

Original languageEnglish (US)
Pages (from-to)6957-6961
Number of pages5
JournalAntimicrobial agents and chemotherapy
Volume60
Issue number11
DOIs
StatePublished - Nov 1 2016

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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