Evaluation of a multiplex PCR assay to rapidly detect Enterobacteriaceae with a broad range of β-lactamases directly from perianal swabs

Kalyan D. Chavda, Michael J. Satlin, Liang Chen, Claudia Manca, Stephen G. Jenkins, Thomas J. Walsh, Barry N. Kreiswirth

Research output: Contribution to journalArticle

Abstract

We developed and evaluated multiplexed molecular beacon probes in a real-time PCR assay to identify prominent extended-spectrum-β-lactamase, plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemase genes directly from perianal swab specimens within 6 h. We evaluated this assay on 158 perianal swabs collected from hematopoietic stem cell transplant recipients and found that this assay was highly sensitive and specific for detection of CTX-M-, pAmpC-, and KPC-producing Enterobacteriaceae compared to culture on chromogenic agar.

Original languageEnglish (US)
Pages (from-to)6957-6961
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume60
Issue number11
DOIs
StatePublished - Nov 1 2016
Externally publishedYes

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Multiplex Polymerase Chain Reaction
Enterobacteriaceae
Plasmids
Molecular Probes
Hematopoietic Stem Cells
Agar
Real-Time Polymerase Chain Reaction
Transplants
Genes
carbapenemase
Transplant Recipients

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Evaluation of a multiplex PCR assay to rapidly detect Enterobacteriaceae with a broad range of β-lactamases directly from perianal swabs. / Chavda, Kalyan D.; Satlin, Michael J.; Chen, Liang; Manca, Claudia; Jenkins, Stephen G.; Walsh, Thomas J.; Kreiswirth, Barry N.

In: Antimicrobial Agents and Chemotherapy, Vol. 60, No. 11, 01.11.2016, p. 6957-6961.

Research output: Contribution to journalArticle

Chavda, Kalyan D. ; Satlin, Michael J. ; Chen, Liang ; Manca, Claudia ; Jenkins, Stephen G. ; Walsh, Thomas J. ; Kreiswirth, Barry N. / Evaluation of a multiplex PCR assay to rapidly detect Enterobacteriaceae with a broad range of β-lactamases directly from perianal swabs. In: Antimicrobial Agents and Chemotherapy. 2016 ; Vol. 60, No. 11. pp. 6957-6961.
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