Evaluation of a DNA probe to identify enteroaggregative Escherichia coli from children with diarrhoea in Bangladesh.

S. M. Faruque, K. Haider, M. M. Rahman, A. R. Abdul Alim, Abdullah Baqui, Q. S. Ahmad, K. M. Hossain, M. J. Albert

Research output: Contribution to journalArticle

Abstract

Six hundred and seventy-five Escherichia coli isolates obtained from 225 diarrhoeal children less than five years of age were tested for adherence to HeLa cells and for hybridisation with DNA probes for genes conferring aggregative adherence (AggA), localised adherence (LA) and diffuse adherence (DA) to assess the usefulness of a recently developed DNA probe for AggA of enteroaggregative E. coli (EAggEC). The strains were further analysed with the DNA probes for heat--labile enterotoxin (LT), heat--stable enterotoxin (ST), Shiga--like toxins (SLT I and SLT II) and for enteroinvasiveness and adherent strains were all negative for these properties. The HeLa cell assay and DNA probe assays showed excellent agreement in identifying LA and DA positive isolates. However, significant disparities occurred in the case of AggA positive isolates, and the DNA probe failed to identify 31.9% (15 of 47) of the EAggEC identified by the HeLa cell adherence assay. The failure of the DNA probe to identify all the EAggEC indicated that there may be a high degree of genetic heterogeneity for the expression of AggA, and development of more DNA probes is necessary to detect all the possible genetic variants of EAggEC.

Original languageEnglish (US)
Pages (from-to)31-34
Number of pages4
JournalJournal of Diarrhoeal Diseases Research
Volume10
Issue number1
StatePublished - Mar 1992
Externally publishedYes

Fingerprint

Bangladesh
DNA probes
DNA Probes
Diarrhea
diarrhea
Escherichia coli
Shiga Toxin 1
HeLa Cells
enterotoxins
assays
Shiga-like toxin 1
Shiga-like toxins
Shiga-like toxin 2
Hot Temperature
Shiga Toxin 2
heat
cells
Genetic Heterogeneity
Enterotoxins
hybridization

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Faruque, S. M., Haider, K., Rahman, M. M., Abdul Alim, A. R., Baqui, A., Ahmad, Q. S., ... Albert, M. J. (1992). Evaluation of a DNA probe to identify enteroaggregative Escherichia coli from children with diarrhoea in Bangladesh. Journal of Diarrhoeal Diseases Research, 10(1), 31-34.

Evaluation of a DNA probe to identify enteroaggregative Escherichia coli from children with diarrhoea in Bangladesh. / Faruque, S. M.; Haider, K.; Rahman, M. M.; Abdul Alim, A. R.; Baqui, Abdullah; Ahmad, Q. S.; Hossain, K. M.; Albert, M. J.

In: Journal of Diarrhoeal Diseases Research, Vol. 10, No. 1, 03.1992, p. 31-34.

Research output: Contribution to journalArticle

Faruque, SM, Haider, K, Rahman, MM, Abdul Alim, AR, Baqui, A, Ahmad, QS, Hossain, KM & Albert, MJ 1992, 'Evaluation of a DNA probe to identify enteroaggregative Escherichia coli from children with diarrhoea in Bangladesh.', Journal of Diarrhoeal Diseases Research, vol. 10, no. 1, pp. 31-34.
Faruque, S. M. ; Haider, K. ; Rahman, M. M. ; Abdul Alim, A. R. ; Baqui, Abdullah ; Ahmad, Q. S. ; Hossain, K. M. ; Albert, M. J. / Evaluation of a DNA probe to identify enteroaggregative Escherichia coli from children with diarrhoea in Bangladesh. In: Journal of Diarrhoeal Diseases Research. 1992 ; Vol. 10, No. 1. pp. 31-34.
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