Evaluation of a commercially available ELISA assay for detection of Giardia lamblia in fecal specimens

William E. Aldeen, DeVon Hale, Alexander J. Robison, Karen C Carroll

Research output: Contribution to journalArticle

Abstract

A total of 417 fecal samples preserved in 10% buffered formalin and PVA were submitted to a commercial microbiology laboratory only for the detection of Giardia lamblia. Results from fecal specimens collected from 411 patients with gastrointestinal symptoms were compared using the following methods: (a) standard Ova & Parasite (O&P) concentration; (b) Alexon's ProspecT/Giardia enzyme-linked immunosorbent assay (ELISA) test, and (c) Meridian's Direct Fluorescent Antibody (DFA) Stain. In the 29 specimens in which G. lamblia was detected, 10 were O&P, DFA and ELISA positive, 17 were only ELISA positive and two were only Ova & Parasite and Direct Fluorescent Antibody positive. Of the 29 positive specimens, 22 were confirmed as true positives. The ELISA sensitivity was 91% and the specificity was 98%. The expense associated with these methods to detect the presence of Giardia is $11.00, $8.95, and $12.80, respectively. In symptomatic patients, the ProspecT/Giardia ELISA is a cost-effective, rapid, and sensitive method for detecting the presence of G. lamblia in fecal specimens.

Original languageEnglish (US)
Pages (from-to)77-79
Number of pages3
JournalDiagnostic Microbiology and Infectious Disease
Volume21
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Giardia lamblia
Giardia
Enzyme-Linked Immunosorbent Assay
Ovum
Antibodies
Parasites
Meridians
Microbiology
Formaldehyde
Coloring Agents
Costs and Cost Analysis

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

Evaluation of a commercially available ELISA assay for detection of Giardia lamblia in fecal specimens. / Aldeen, William E.; Hale, DeVon; Robison, Alexander J.; Carroll, Karen C.

In: Diagnostic Microbiology and Infectious Disease, Vol. 21, No. 2, 1995, p. 77-79.

Research output: Contribution to journalArticle

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abstract = "A total of 417 fecal samples preserved in 10{\%} buffered formalin and PVA were submitted to a commercial microbiology laboratory only for the detection of Giardia lamblia. Results from fecal specimens collected from 411 patients with gastrointestinal symptoms were compared using the following methods: (a) standard Ova & Parasite (O&P) concentration; (b) Alexon's ProspecT/Giardia enzyme-linked immunosorbent assay (ELISA) test, and (c) Meridian's Direct Fluorescent Antibody (DFA) Stain. In the 29 specimens in which G. lamblia was detected, 10 were O&P, DFA and ELISA positive, 17 were only ELISA positive and two were only Ova & Parasite and Direct Fluorescent Antibody positive. Of the 29 positive specimens, 22 were confirmed as true positives. The ELISA sensitivity was 91{\%} and the specificity was 98{\%}. The expense associated with these methods to detect the presence of Giardia is $11.00, $8.95, and $12.80, respectively. In symptomatic patients, the ProspecT/Giardia ELISA is a cost-effective, rapid, and sensitive method for detecting the presence of G. lamblia in fecal specimens.",
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