Evaluating the effects of subclinical, cyclic ischemia-reperfusion injury on wound healing using a novel device in the rabbit ear

Jordan P. Steinberg, Anandev N. Gurjala, Shengxian Jia, Seok Jong Hong, Robert D. Galiano, Thomas A. Mustoe

Research output: Contribution to journalArticle

Abstract

Objective: This study aimed to evaluate the effect of cyclic ischemia-reperfusion (IR) injury on wound healing using a novel rabbit ear model. Materials and Methods: A lightweight clamp apparatus was developed for reversible occlusion of the central ear artery. Ventral ear wounds were analyzed postoperatively for epithelialization and granulation as well as gene expression after 3 consecutive days of IR cycling. Results: By postoperative day #7, ears showed no gross tissue necrosis, but histologic analysis of wounds confirmed a significant impairment in epithelial and granulation tissue gaps as well as total epithelial and granulation tissue areas (P < 0.001). Quantitative polymerase chain reaction analysis of IR wounds indicated significant up-regulation of heat shock protein-70 and downregulation of superoxide dismutase 1 relative to sham controls (P < 0.05). Conclusions: A novel rabbit ear model for the induction of subclinical, cyclic IR injury in cutaneous tissue has been developed that will serve as a valuable tool for the testing of new therapeutics.

Original languageEnglish (US)
Pages (from-to)698-705
Number of pages8
JournalAnnals of plastic surgery
Volume72
Issue numberSUPPL. 2
DOIs
StatePublished - Jun 2014

Keywords

  • Chronic wounds
  • Ischemia
  • Ischemia-reperfusion injury
  • Rabbit ear
  • Reperfusion injury

ASJC Scopus subject areas

  • Surgery

Fingerprint Dive into the research topics of 'Evaluating the effects of subclinical, cyclic ischemia-reperfusion injury on wound healing using a novel device in the rabbit ear'. Together they form a unique fingerprint.

  • Cite this