Evaluating shigella flexneri pathogenesis in the human enteroid model

Sridevi Ranganathan, Michele Doucet, Christen L. Grassel, Bre Onna Delaine-Elias, Nicholas Zachos, Eileen M. Barry

Research output: Contribution to journalArticle

Abstract

The enteric pathogen Shigella is one of the leading causes of moderate-to-severe diarrhea and death in young children in developing countries. Transformed cell lines and animal models have been widely used to study Shigella pathogenesis. In addition to altered physiology, transformed cell lines are composed of a single cell type that does not sufficiently represent the complex multicellular environment of the human colon. Most available animal models do not accurately mimic human disease. The human intestinal enteroid model, derived from LGR5 stem cell-containing intestinal crypts from healthy subjects, represents a technological leap in human gastrointestinal system modeling and provides a more physiologically relevant system that includes multiple cell types and features of the human intestine. We established the utility of this model for studying basic aspects of Shigella pathogenesis and host responses. In this study, we show that Shigella flexneri is capable of infecting and replicating intracellularly in human enteroids derived from different segments of the intestine. Apical invasion by S. flexneri is very limited but increases 10-fold when enteroids are differentiated to include M cells. Invasion via the basolateral surface was at least 2-log 10 units more efficient than apical infection. Increased secretion of interleukin-8 and higher expression levels of the mucin glycoprotein Muc2 were observed in the enteroids following S. flexneri infection. The human enteroid model promises to bridge some of the gaps between traditional cell culture, animal models, and human infection.

Original languageEnglish (US)
Article numbere00740
JournalInfection and Immunity
Volume87
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Shigella flexneri
Shigella
Transformed Cell Line
Animal Models
Intestines
Infection
Mucins
Interleukin-8
Developing Countries
Diarrhea
Glycoproteins
Healthy Volunteers
Colon
Stem Cells
Cell Culture Techniques

Keywords

  • Colonoid
  • Human intestinal enteroids
  • M cells
  • S. flexneri pathogenesis

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Ranganathan, S., Doucet, M., Grassel, C. L., Delaine-Elias, B. O., Zachos, N., & Barry, E. M. (2019). Evaluating shigella flexneri pathogenesis in the human enteroid model. Infection and Immunity, 87(4), [e00740]. https://doi.org/10.1128/IAI.00740-18

Evaluating shigella flexneri pathogenesis in the human enteroid model. / Ranganathan, Sridevi; Doucet, Michele; Grassel, Christen L.; Delaine-Elias, Bre Onna; Zachos, Nicholas; Barry, Eileen M.

In: Infection and Immunity, Vol. 87, No. 4, e00740, 01.04.2019.

Research output: Contribution to journalArticle

Ranganathan, S, Doucet, M, Grassel, CL, Delaine-Elias, BO, Zachos, N & Barry, EM 2019, 'Evaluating shigella flexneri pathogenesis in the human enteroid model', Infection and Immunity, vol. 87, no. 4, e00740. https://doi.org/10.1128/IAI.00740-18
Ranganathan S, Doucet M, Grassel CL, Delaine-Elias BO, Zachos N, Barry EM. Evaluating shigella flexneri pathogenesis in the human enteroid model. Infection and Immunity. 2019 Apr 1;87(4). e00740. https://doi.org/10.1128/IAI.00740-18
Ranganathan, Sridevi ; Doucet, Michele ; Grassel, Christen L. ; Delaine-Elias, Bre Onna ; Zachos, Nicholas ; Barry, Eileen M. / Evaluating shigella flexneri pathogenesis in the human enteroid model. In: Infection and Immunity. 2019 ; Vol. 87, No. 4.
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