Abstract
Directed accessibility mediated by antigen-receptor gene enhancers ensures developmental stage-specific activation of V(D)J recombination. Here, we used a combination of in vitro and in vivo assays to explore the mechanisms that regulate immunoglobulin μ heavy chain gene enhancer- dependent chromatin accessibility. Ets-1 or PU.1 bound to μ enhancer- containing plasmids assembled into chromatin in vitro and increased restriction enzyme access to a proximal site. In complementary analyses, expression of PU.1 in Ets-1-containing 2017 pro-T cells or NIH 3T3 cells induced sterile Iμ transcripts at the IgH locus and increased accessibility of the endogenous μ enhancer to restriction endonucleases. These observations suggest that one role of PU.1 is to increase accessibility of the μ locus to initiate heavy chain gene expression.
Original language | English (US) |
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Pages (from-to) | 11-20 |
Number of pages | 10 |
Journal | Immunity |
Volume | 11 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1999 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
- Immunology