ETS protein-dependent accessibility changes at the immunoglobulin μ heavy chain enhancer

Barbara S. Nikolajczyk, J. Aquiles Sanchez, Ranjan Sen

Research output: Contribution to journalArticle

Abstract

Directed accessibility mediated by antigen-receptor gene enhancers ensures developmental stage-specific activation of V(D)J recombination. Here, we used a combination of in vitro and in vivo assays to explore the mechanisms that regulate immunoglobulin μ heavy chain gene enhancer- dependent chromatin accessibility. Ets-1 or PU.1 bound to μ enhancer- containing plasmids assembled into chromatin in vitro and increased restriction enzyme access to a proximal site. In complementary analyses, expression of PU.1 in Ets-1-containing 2017 pro-T cells or NIH 3T3 cells induced sterile Iμ transcripts at the IgH locus and increased accessibility of the endogenous μ enhancer to restriction endonucleases. These observations suggest that one role of PU.1 is to increase accessibility of the μ locus to initiate heavy chain gene expression.

Original languageEnglish (US)
Pages (from-to)11-20
Number of pages10
JournalImmunity
Volume11
Issue number1
DOIs
StatePublished - Jul 1999
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

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