TY - JOUR
T1 - Ethnic, geographic, and genetic differences in arsenic metabolism at low arsenic exposure
T2 - A preliminary analysis in the multi-ethnic study of atherosclerosis (mesa)
AU - Balakrishnan, Poojitha
AU - Jones, Miranda R.
AU - Vaidya, Dhananjay
AU - Tellez-Plaza, Maria
AU - Post, Wendy S.
AU - Kaufman, Joel D.
AU - Bielinski, Suzette J.
AU - Taylor, Kent
AU - Francesconi, Kevin
AU - Goessler, Walter
AU - Navas-Acien, Ana
N1 - Publisher Copyright:
© 2018, MDPI AG. All rights reserved.
PY - 2018/6/5
Y1 - 2018/6/5
N2 - We investigated the effect of candidate variants in AS3MT (arsenic (III) methyltransferase) with urinary arsenic metabolites and their principal components in a subset of 264 participants in the Multi-Ethnic Study of Atherosclerosis (MESA). Urinary arsenic species, including inorganic arsenic (iAs), monomethylarsonate (MMA), dimethylarsinate (DMA), and arsenobetaine (Ab), were measured using high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS) and corrected for organic sources from seafood consumption by regressing Ab on arsenic species using a validated method. Principal components of arsenic metabolism were also used as independent phenotypes. We conducted linear regression of arsenic traits with allelic dosage of candidate single nucleotide polymorphisms (SNPs) rs12768205 (G > A), rs3740394 (A > G), and rs3740393 (G > C) measured using Illumina MetaboChip. Models were stratified by non-Hispanic white vs. all other race/ethnicity and adjusted for age, sex, arsenic exposure, study site, and population stratification. Consistent with previous studies, rs12768205 showed evidence for strongest association (non-Hispanic white: iAs% −0.14 (P 0.83), MMA% −0.66 (0.49), DMA% 0.81(0.49); other race/ethnicity: 0.13 (0.71), −1.21 (0.09), 1.08 (0.20)). No association, however, passed the strict Bonferroni p-value. This was a novel study among an ethnically diverse population exposed to low arsenic levels.
AB - We investigated the effect of candidate variants in AS3MT (arsenic (III) methyltransferase) with urinary arsenic metabolites and their principal components in a subset of 264 participants in the Multi-Ethnic Study of Atherosclerosis (MESA). Urinary arsenic species, including inorganic arsenic (iAs), monomethylarsonate (MMA), dimethylarsinate (DMA), and arsenobetaine (Ab), were measured using high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS) and corrected for organic sources from seafood consumption by regressing Ab on arsenic species using a validated method. Principal components of arsenic metabolism were also used as independent phenotypes. We conducted linear regression of arsenic traits with allelic dosage of candidate single nucleotide polymorphisms (SNPs) rs12768205 (G > A), rs3740394 (A > G), and rs3740393 (G > C) measured using Illumina MetaboChip. Models were stratified by non-Hispanic white vs. all other race/ethnicity and adjusted for age, sex, arsenic exposure, study site, and population stratification. Consistent with previous studies, rs12768205 showed evidence for strongest association (non-Hispanic white: iAs% −0.14 (P 0.83), MMA% −0.66 (0.49), DMA% 0.81(0.49); other race/ethnicity: 0.13 (0.71), −1.21 (0.09), 1.08 (0.20)). No association, however, passed the strict Bonferroni p-value. This was a novel study among an ethnically diverse population exposed to low arsenic levels.
KW - AS3MT
KW - Arsenic
KW - Epidemiology
KW - Genetic susceptibility
KW - Geography
KW - MESA
KW - Methylation
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U2 - 10.3390/ijerph15061179
DO - 10.3390/ijerph15061179
M3 - Article
C2 - 29874848
AN - SCOPUS:85048268749
SN - 1661-7827
VL - 15
JO - International journal of environmental research and public health
JF - International journal of environmental research and public health
IS - 6
M1 - 1179
ER -