Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry

Aurelie Roux, Shelley N. Jackson, Ludovic Muller, Damon Barbacci, Joseph O'Rourke, Panayotis K. Thanos, Nora D. Volkow, Carey Balaban, J. Albert Schultz, Amina S. Woods

Research output: Contribution to journalArticle

Abstract

Alcohol abuse is a chronic disease characterized by the consumption of alcohol at a level that interferes with physical and mental health and causes serious and persistent changes in the brain. Lipid metabolism is of particular interest due to its high concentration in the brain. Lipids are the main component of cell membranes, are involved in cell signaling, signal transduction, and energy storage. In this study, we analyzed lipid composition of chronically ethanol exposed mouse brains. Juvenile (JUV) and adult (ADU) mice were placed on a daily limited-access ethanol intake model for 52 days. After euthanasia, brains were harvested, and total lipids were extracted from brain homogenates. Samples were analyzed using high resolution mass spectrometry and processed by multivariate and univariate statistical analysis. Significant lipid changes were observed in different classes including sphingolipids, fatty acids, lysophosphatidylcholines, and other glycerophospholipids.

Original languageEnglish (US)
Pages (from-to)1148-1156
Number of pages9
JournalACS Chemical Neuroscience
Volume7
Issue number8
DOIs
StatePublished - Aug 17 2016
Externally publishedYes

Keywords

  • alcohol
  • electrospray ionization
  • lipids
  • mass spectrometry
  • solid-phase extraction

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Cognitive Neuroscience

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  • Cite this

    Roux, A., Jackson, S. N., Muller, L., Barbacci, D., O'Rourke, J., Thanos, P. K., Volkow, N. D., Balaban, C., Schultz, J. A., & Woods, A. S. (2016). Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry. ACS Chemical Neuroscience, 7(8), 1148-1156. https://doi.org/10.1021/acschemneuro.6b00120