Ethanol increases the formation of NADP+ in rat hepatocytes

B. Emmanuel Akinshola, James J. Potter, Esteban Mezey

Research output: Contribution to journalArticle

Abstract

The acute effects of ethanol on total (bound + free) pyridine dinucleotides were determined in freshly isolated rat hepatocytes. Pyridine dinucleotides and adenine nucleotides were determined by highperformance liquid chromatography. Exposure of the hepatocytes to 8 mmol/L ethanol resulted in a decrease in NAD+ and an increase in NADP+ after 2 min incubation. There were no significant changes in NADH and NADPH. Ethanol decreased ATP and increased AMP after 2 min, whereas an increase in ADP was only apparent after 15 min of incubation. Ethanol 8 mmol/L and 100 mmol/L resulted in an increased incorporation of [32P] into NADP+ from [32P]‐prelabeled NAD+ and ATP. Ethanol increased hepatocyte NAD+ kinase activity; this effect was blocked by 4‐methylpyrazole but reproduced by 10 μmol acetaldehyde. These observations indicate that ethanol increases the synthesis of NADP+ and that this effect is most likely the result of increased NAD+ kinase activity. The ethanol‐induced decrease of NAD+ may limit ADP ribosylation of nuclear proteins, whereas increases in NADP+ may stimulate the pentose phosphate cycle. (HEPATOLOGY 1991;13:509–14.)

Original languageEnglish (US)
Pages (from-to)509-514
Number of pages6
JournalHepatology
Volume13
Issue number3
DOIs
StatePublished - Mar 1991

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ASJC Scopus subject areas

  • Hepatology

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