Estrogen-responsive transient expression assay using a brain aromatase-based reporter gene in zebrafish (Danio rerio)

Dong Jae Kim, Seung Hyeok Seok, Min Won Baek, Hui Young Lee, Yi Rang Na, Sung Hoon Park, Hyun Kyoung Lee, Noton Dutta, Koichi Kawakami, Jae Hak Park

Research output: Contribution to journalArticle

Abstract

Whereas endogenous estrogens play an important role in the development, maintenance, and function of female and male reproductive organs, xenoestrogens present in the environment disrupt normal endocrine function in humans and wildlife. Various in vivo and in vitro assays have been developed to screen these xenoestrogens. However, traditional in vivo assays are laborious and unsuitable for large-scale screening, and in vitro assays do not necessarily replicate in vivo functioning. To overcome these limitations, we developed a transient expression assay in zebrafish, into which a brain aromatase (cyp19a1b)-based estrogen-responsive reporter gene was introduced. In response to 17β-estradiol (10-6 M) and heptachlor (10-6 M), zebrafish embryos carrying the reporter construct expressed enhanced green fluorescent protein in the olfactory bulb, telencephalon, preoptic area, and mediobasal hypothalamus. This system will serve to model the in vivo conversion and breakdown of estrogenic compounds and thus provide a rapid preliminary screening method to estimate their estrogenicity.

Original languageEnglish (US)
Pages (from-to)416-423
Number of pages8
JournalComparative Medicine
Volume59
Issue number5
Publication statusPublished - Oct 2009
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Kim, D. J., Seok, S. H., Baek, M. W., Lee, H. Y., Na, Y. R., Park, S. H., ... Park, J. H. (2009). Estrogen-responsive transient expression assay using a brain aromatase-based reporter gene in zebrafish (Danio rerio). Comparative Medicine, 59(5), 416-423.