Estrogen receptor alpha overexpressing mouse antral follicles are sensitive to atresia induced by methoxychlor and its metabolites

Tessie Paulose, Patrick R. Hannon, Jackye Peretz, Zelieann R. Craig, Jodi A. Flaws

Research output: Contribution to journalArticlepeer-review

Abstract

Methoxychlor (MXC) and its metabolites bind to estrogen receptors (ESRs) and increase ovarian atresia. To test whether ESR alpha (ESR1) overexpressing (ESR1 OE) antral follicles are more sensitive to atresia compared to controls, we cultured antral follicles with vehicle, MXC (1-100. μg/ml) or metabolites (0.1-10. μg/ml). Results indicate that MXC and its metabolites significantly increase atresia in ESR1 OE antral follicles at lower doses compared to controls. Activity of pro-apoptotic factor caspase-3/7 was significantly higher in ESR1 OE treated antral follicles compared to controls. ESR1 OE mice dosed with MXC 64. mg/kg/day had an increased percentage of atretic antral follicles compared to controls. Furthermore, pro-caspase-3 levels were found to be significantly lower in ESR1 OE ovaries than controls dosed with MXC 64. mg/kg/day. These data suggest that ESR1 OE ovaries are more sensitive to atresia induced by MXC and its metabolites . in vitro and . in vivo compared to controls.

Original languageEnglish (US)
Pages (from-to)353-360
Number of pages8
JournalReproductive Toxicology
Volume33
Issue number3
DOIs
StatePublished - Jun 2012
Externally publishedYes

Keywords

  • Antral follicles
  • Apoptosis
  • Atresia
  • Estrogenic
  • Metabolites
  • Methoxychlor
  • Reproduction

ASJC Scopus subject areas

  • Toxicology

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