TY - JOUR
T1 - Estrogen receptor α induces prosurvival autophagy in papillary thyroid cancer via stimulating reactive oxygen species and extracellular signal regulated kinases
AU - Fan, Dahua
AU - Liu, Shirley Y.W.
AU - Van Hasselt, C. Andrew
AU - Vlantis, Alexander C.
AU - Ng, Enders K.W.
AU - Zhang, Haitao
AU - Dong, Yujuan
AU - Ng, Siu Kwan
AU - Chu, Ryan
AU - Chan, Amy B.W.
AU - Du, Jing
AU - Wei, Wei
AU - Liu, Xiaoling
AU - Liu, Zhimin
AU - Xing, Mingzhao
AU - Chen, George G.
N1 - Publisher Copyright:
Copyright © 2015 by the Endocrine Society.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Context: The incidence of papillary thyroid cancer (PTC) shows a predominance in females, with a male: female ratio of 1:3, and none of the known risk factors are associated with gender difference. Increasing evidence indicates a role of estrogen in thyroid tumorigenesis, but the mechanism involved remains largely unknown. Objective: This study aimed to assess the contribution of autophagy to estrogen receptor α (ERα)-mediated growth of PTC. Design: The expression of ERα in thyroid tissue of patients with PTC tissues was analyzed. Cell viability, proliferation, and apoptosis were evaluated after chemical and genetic inhibition of autophagy. Autophagy in PTC cell lines BCPAP and BCPAP-ERα was assessed. Results: ERα expression was increased in PTC tissues compared with the adjacent nontumor tissues. Estrogen induced autophagy in an ERα-dependent manner. Autophagy induced by estrogen/ERα is associated with generation of reactive oxygen species, activation of ERK1/2, and the survival/growth of PTC cells. Chemical and genetic inhibition of autophagy dramatically decreased tumor cell survival and promoted apoptosis, confirming the positive role of autophagy in the growth of PTC. Conclusions: ERα contributes to the growth of PTC by enhancinganimportant prosurvival catabolic process, autophagy, in PTC cells. The inhibition of autophagy promotes apoptosis, implicating a novel strategy for the treatment of ERα-positive PTC.
AB - Context: The incidence of papillary thyroid cancer (PTC) shows a predominance in females, with a male: female ratio of 1:3, and none of the known risk factors are associated with gender difference. Increasing evidence indicates a role of estrogen in thyroid tumorigenesis, but the mechanism involved remains largely unknown. Objective: This study aimed to assess the contribution of autophagy to estrogen receptor α (ERα)-mediated growth of PTC. Design: The expression of ERα in thyroid tissue of patients with PTC tissues was analyzed. Cell viability, proliferation, and apoptosis were evaluated after chemical and genetic inhibition of autophagy. Autophagy in PTC cell lines BCPAP and BCPAP-ERα was assessed. Results: ERα expression was increased in PTC tissues compared with the adjacent nontumor tissues. Estrogen induced autophagy in an ERα-dependent manner. Autophagy induced by estrogen/ERα is associated with generation of reactive oxygen species, activation of ERK1/2, and the survival/growth of PTC cells. Chemical and genetic inhibition of autophagy dramatically decreased tumor cell survival and promoted apoptosis, confirming the positive role of autophagy in the growth of PTC. Conclusions: ERα contributes to the growth of PTC by enhancinganimportant prosurvival catabolic process, autophagy, in PTC cells. The inhibition of autophagy promotes apoptosis, implicating a novel strategy for the treatment of ERα-positive PTC.
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U2 - 10.1210/jc.2014-3257
DO - 10.1210/jc.2014-3257
M3 - Article
C2 - 25594859
AN - SCOPUS:84927645586
SN - 0021-972X
VL - 100
SP - E561-E571
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 4
ER -