Abstract
ClC-2 is a CLC family member of chloride channels sensitive to changes in cell volume, pH and voltage. The ClC-2 is widely distributed along the nephron although in the kidney its role still not well understood. Aldosterone studies suggest that ClC-2 expression in the kidney may be hormonally regulated. To explore the possibility that estrogen control ClC-2 expression, we investigated whether its expression changed in the kidney of female Wistar rats subjected to ovariectomy with or without near-physiological or high doses of 17β-estradiol benzoate treatment for 10 days. Total RNA isolated from rat kidney and dissected nephron segments was analyzed by ribonuclease protection assay and/or a semi-quantitative RT-PCR. The renal ClC-2 protein expression was analyzed by Western blot. The decreased renal expression of ClC-2 mRNA and protein observed in ovariectomized rats was restored to control levels after treatment with low doses of estradiol. Higher dose estradiol lead to an even greater increase in ClC-2 mRNA and protein expression. This change in overall expression was shown to be caused by the modulation of ClC-2 mRNA expression in the proximal tubule. These results suggest that ClC-2 may be involved in estrogen-induced Cl- transport in rat kidney.
Original language | English (US) |
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Pages (from-to) | 593-599 |
Number of pages | 7 |
Journal | Pflugers Archiv European Journal of Physiology |
Volume | 446 |
Issue number | 5 |
DOIs | |
State | Published - Aug 1 2003 |
Keywords
- Chloride channel
- ClC-2 mRNA
- Estrogen
- Kidney
- Nephron
- Polymerase chain reaction
- Rat
- Ribonuclease protection assay
ASJC Scopus subject areas
- Physiology
- Clinical Biochemistry
- Physiology (medical)