Estrogen modulates CIC-2 chloride channel gene expression in rat kidney

Danielle S. Nascimento, Carlos U. Reis, Regina C. Goldenberg, Tânia M. Ortiga-Carvalho, Carmen C. Pazos-Moura, Sandra E. Guggino, William B. Guggino, Marcelo M. Morales

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


ClC-2 is a CLC family member of chloride channels sensitive to changes in cell volume, pH and voltage. The ClC-2 is widely distributed along the nephron although in the kidney its role still not well understood. Aldosterone studies suggest that ClC-2 expression in the kidney may be hormonally regulated. To explore the possibility that estrogen control ClC-2 expression, we investigated whether its expression changed in the kidney of female Wistar rats subjected to ovariectomy with or without near-physiological or high doses of 17β-estradiol benzoate treatment for 10 days. Total RNA isolated from rat kidney and dissected nephron segments was analyzed by ribonuclease protection assay and/or a semi-quantitative RT-PCR. The renal ClC-2 protein expression was analyzed by Western blot. The decreased renal expression of ClC-2 mRNA and protein observed in ovariectomized rats was restored to control levels after treatment with low doses of estradiol. Higher dose estradiol lead to an even greater increase in ClC-2 mRNA and protein expression. This change in overall expression was shown to be caused by the modulation of ClC-2 mRNA expression in the proximal tubule. These results suggest that ClC-2 may be involved in estrogen-induced Cl- transport in rat kidney.

Original languageEnglish (US)
Pages (from-to)593-599
Number of pages7
JournalPflugers Archiv European Journal of Physiology
Issue number5
StatePublished - Aug 1 2003


  • Chloride channel
  • ClC-2 mRNA
  • Estrogen
  • Kidney
  • Nephron
  • Polymerase chain reaction
  • Rat
  • Ribonuclease protection assay

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)


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