Estrogen induces retinoid receptor expression in mouse cervical epithelia

Giulia Celli, Nadine Darwiche, Luigi M De Luca

Research output: Contribution to journalArticle

Abstract

Steroid hormones and retinoids are powerful regulators of normal epithelial differentiation and function in the mouse female reproductive tract, where their actions are mediated by nuclear receptors. The expression pattern, heterodimeric interactions, and availability of ligand of these transcription factors are thought to contribute to the biological response. Estrogen (E2) induces proliferation and squamous differentiation of the cervical and vaginal epithelium during the mouse estrous cycle, while progesterone and retinoids maintain the simple columnar epithelium of the endocervix and uterine horns. We wanted to investigate whether retinoid receptors are responsive to estrogen status during cervical epithelial differentiation induced by a single dose of estrogen in ovariectomized adult mice. Northern blot analysis demonstrated a prolonged induction of RXRα and RARγ gene expression by E2 in the mouse cervix and vagina. When the induction of RXRα and RARγ was compared to genes known to be responsive to E2, including estrogen receptor (ER) and c-fos, RXRα was induced within 0.5 h of hormone treatment, while RARγ induction was evident by 4 h. The induction of these retinoid receptors suggests that they may be implicated in epithelial growth and differentiation in response to E2. Moreover, potential heterodimeric interactions among these receptors indicate that normal, cyclical epithelial differentiation results from the interplay of these molecules. Using in situ hybridization analysis, RXRα transcripts were localized preferentially in the basal cells, while ER mRNA was expressed throughout the epithelium of the ectocervix and vagina. Furthermore, ER transcripts were highly expressed in the stratified squamous foci induced by mild vitamin A depletion in the columnar epithelium of the endocervix and uterine horns. Therefore, the induction of RXRα and RARγ by E2 and their expression pattern in relation to ER suggest that they are needed to coordinate specific genetic programs that result in cervical epithelial growth and differentiation.

Original languageEnglish (US)
Pages (from-to)273-282
Number of pages10
JournalExperimental Cell Research
Volume226
Issue number2
DOIs
StatePublished - Aug 1 1996
Externally publishedYes

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Retinoids
Estrogen Receptors
Estrogens
Epithelium
Vagina
Hormones
Estrous Cycle
Cytoplasmic and Nuclear Receptors
Growth
Vitamin A
Cervix Uteri
Northern Blotting
In Situ Hybridization
Progesterone
Transcription Factors
Steroids
Ligands
Gene Expression
Messenger RNA
Genes

ASJC Scopus subject areas

  • Cell Biology

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Estrogen induces retinoid receptor expression in mouse cervical epithelia. / Celli, Giulia; Darwiche, Nadine; De Luca, Luigi M.

In: Experimental Cell Research, Vol. 226, No. 2, 01.08.1996, p. 273-282.

Research output: Contribution to journalArticle

Celli, Giulia ; Darwiche, Nadine ; De Luca, Luigi M. / Estrogen induces retinoid receptor expression in mouse cervical epithelia. In: Experimental Cell Research. 1996 ; Vol. 226, No. 2. pp. 273-282.
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