Estrogen-enhanced expression of bcl-2 mRNA in rat brain after experimental stroke

N. J. Alkayed, S. K. McCune, B. J. Crain, R. J. Traystman, P. D. Hurn

Research output: Contribution to journalArticle

Abstract

We have previously demonstrated that estrogen protects neural tissue from ischemic damage. Estrogen has been shown to increase anti-apoptptic bcl-2 gene expression in nonneural tissue. Bcl-2 is expressed in brain and has been shown to promote neuronal cell survival. Thus, we hypothesized that estrogen enhances expression of bcl-2 after ischemic brain injury. Age-matched male (M), intact female (F), ovariectomized female (O) and estrogen-supplemented ovariectomized (E, via 25-μg slow-release pellets) Wistar rats were subjected to 2 hours of intraluminal middle cerebral artery occlusion, followed by 22 hours of reperfusion. Arterial blood pressure and gases, and rectal and temporalis muscle temperatures were controlled, and laser-Doppler cerebral blood flow (LDF) was monitored to confirm ischemic status. In-situ hybridization of brain slices was performed with a radiolabeled antisense bcl-2 oligonucleotide probe. Control hybridizations were performed with sense and excess unlabeled antisense probes. Slices were apposed to film for 3 wks, covered with emulsion for 6 wks and counterstained with Thionin for histological examination. Bcl-2 signal was concentrated over cells predominantly in the penumbra. Viable cells were scarce in the infarct core with occasional islands of bcl-2-expressing cells. Autoradiographic density was quantified in ischemic cerebral cortex and basal ganglia and expressed as a percentage of optical density on the contralateral side. The results are summarized in the following table (mean ±sem): Group n # slices Cerebral Cortex Basal Ganglia LDF, % ctrl M 4 64 69 ± 20 55 ± 20 31 ± 4 F 4 47 94 ± 4 68 ± 19 40 ± 1 O 2 35 61 ± 39 52 ± 6 36 ± 2 E 2 28 120 ± 0 90 ± 37 48 ± 5 These findings suggest a role for anti-apoptotic bcl-2 gene expression in estrogen-mediated neuroprotection.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

Fingerprint

stroke
estrogens
Rats
Brain
Estrogens
Stroke
brain
Messenger RNA
rats
bcl-2 Genes
cerebral cortex
Basal Ganglia
Gene expression
Cerebral Cortex
Cerebrovascular Circulation
Thionins
Tissue
Gene Expression
gene expression
oligonucleotide probes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Alkayed, N. J., McCune, S. K., Crain, B. J., Traystman, R. J., & Hurn, P. D. (1998). Estrogen-enhanced expression of bcl-2 mRNA in rat brain after experimental stroke. FASEB Journal, 12(5).

Estrogen-enhanced expression of bcl-2 mRNA in rat brain after experimental stroke. / Alkayed, N. J.; McCune, S. K.; Crain, B. J.; Traystman, R. J.; Hurn, P. D.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Alkayed, NJ, McCune, SK, Crain, BJ, Traystman, RJ & Hurn, PD 1998, 'Estrogen-enhanced expression of bcl-2 mRNA in rat brain after experimental stroke', FASEB Journal, vol. 12, no. 5.
Alkayed NJ, McCune SK, Crain BJ, Traystman RJ, Hurn PD. Estrogen-enhanced expression of bcl-2 mRNA in rat brain after experimental stroke. FASEB Journal. 1998 Mar 20;12(5).
Alkayed, N. J. ; McCune, S. K. ; Crain, B. J. ; Traystman, R. J. ; Hurn, P. D. / Estrogen-enhanced expression of bcl-2 mRNA in rat brain after experimental stroke. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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