Establishment of T-lymphocyte subset reference intervals in a healthy adult population in Chennai, India

K. G. Murugavel, P. Balakrishnan, J. Mohanakrishnan, S. S. Solomon, E. M. Shankar, Sandeep Pulimi, Muthu Sundaram, Nagalingeswaran Kumarasamy, Estelle Piwowar-Manning, Edward Livant, K. H. Mayer, S. P. Thyagarajan, Suniti Solomon

Research output: Contribution to journalArticle

Abstract

Estimation of CD4+ T-lymphocytes continues to be an important aspect for monitoring HIV disease progression and response to antiretroviral therapy. Most of the diagnostic laboratories often rely on western text books for CD4+ T-lymphocyte reference values, which could, often be unreliable for usage in local settings. Therefore, we attempted to establish the reference values for T-lymphocyte subsets among healthy adults in a cross-sectional study carried out at the YRG Centre for AIDS Research and Education (YRG CARE) in Chennai, south India, in 213 (84 female and 129 male) healthy, HIV-1/2 seronegative adults as volunteers. Whole blood specimens were processed for CD4+, CD8+ T-lymphocyte estimation and haematological parameters. The established range of CD4+ T-lymphocyte counts for men and women were 383 - 1347 cells/μl (mean 865 and median 845 cells/μl) and 448 - 1593 cells/μl (mean 1021 and median 954 cells/μl), respectively. Women had significantly higher absolute CD4+ Tlymphocyte counts (P<0.001) and CD4+:CD8+ T-lymphocyte ratio as compared to men. The established normal range of CD4+ T-lymphocyte % was 21 - 59 (mean 40.2 and median 40.1). The influence of age was not observed in any of the parameters except CD4+/CD8+ T-lymphocyte ratio with the >45 yr age group. Further studies with greater sample size may be required to define the staging of HIV disease in relation to the normal CD4 T-lymphocyte count in the general population.

Original languageEnglish (US)
Pages (from-to)59-63
Number of pages5
JournalIndian Journal of Medical Research
Volume129
Issue number1
StatePublished - Jun 1 2009
Externally publishedYes

Keywords

  • CD4+ T-lymphocyte
  • India
  • Reference range

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Murugavel, K. G., Balakrishnan, P., Mohanakrishnan, J., Solomon, S. S., Shankar, E. M., Pulimi, S., Sundaram, M., Kumarasamy, N., Piwowar-Manning, E., Livant, E., Mayer, K. H., Thyagarajan, S. P., & Solomon, S. (2009). Establishment of T-lymphocyte subset reference intervals in a healthy adult population in Chennai, India. Indian Journal of Medical Research, 129(1), 59-63.