Abstract
An immortal cell line of Copenhagen rat prostate epithelium was established after transfection of primary cultured cells with SV40 DNA and designated EPYP-1. The established cells have continued to proliferate more than 70 population doublings, have not undergone senescence, and stain positively for SV40 T-antigen in their nuclei by immunohistochemistry. They grow in a monolayer with an epithelial morphology and exhibit positive staining for cytokeratin. EPYP-1 cells exhibited positive staining with anti-prostatic acid phosphatase antibody and expressed Integrin α6,B1 on their plasma membrane. They did nor demonstrate Dil-Ac-LDL uptake as an endothelial marker. Both testosterone and dihydrotestosterone stimulated the growth of EPYP-1. Genetically EPYP-1 was aneuploid, however no tumors formed after subcutaneous and intra-prostatic injection of the cells in Copenhagen rats. This immortalized cell line of Copenhagen rat prostate epithelial origin may be suitable for studying early events in the conversion of cells to tumorigenicity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 427-431 |
| Number of pages | 5 |
| Journal | In vivo (Athens, Greece) |
| Volume | 9 |
| Issue number | 5 |
| State | Published - 1995 |
| Externally published | Yes |
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Keywords
- Copenhagen rat
- Immortalization
- Prostate cancer
- Prostate epithelium
- T-antigen
ASJC Scopus subject areas
- Medicine(all)
Cite this
Establishment of an immortalized Copenhagen rat prostate epithelial cell line. / Yamazaki, K.; Pienta, Kenneth.
In: In vivo (Athens, Greece), Vol. 9, No. 5, 1995, p. 427-431.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Establishment of an immortalized Copenhagen rat prostate epithelial cell line
AU - Yamazaki, K.
AU - Pienta, Kenneth
PY - 1995
Y1 - 1995
N2 - An immortal cell line of Copenhagen rat prostate epithelium was established after transfection of primary cultured cells with SV40 DNA and designated EPYP-1. The established cells have continued to proliferate more than 70 population doublings, have not undergone senescence, and stain positively for SV40 T-antigen in their nuclei by immunohistochemistry. They grow in a monolayer with an epithelial morphology and exhibit positive staining for cytokeratin. EPYP-1 cells exhibited positive staining with anti-prostatic acid phosphatase antibody and expressed Integrin α6,B1 on their plasma membrane. They did nor demonstrate Dil-Ac-LDL uptake as an endothelial marker. Both testosterone and dihydrotestosterone stimulated the growth of EPYP-1. Genetically EPYP-1 was aneuploid, however no tumors formed after subcutaneous and intra-prostatic injection of the cells in Copenhagen rats. This immortalized cell line of Copenhagen rat prostate epithelial origin may be suitable for studying early events in the conversion of cells to tumorigenicity.
AB - An immortal cell line of Copenhagen rat prostate epithelium was established after transfection of primary cultured cells with SV40 DNA and designated EPYP-1. The established cells have continued to proliferate more than 70 population doublings, have not undergone senescence, and stain positively for SV40 T-antigen in their nuclei by immunohistochemistry. They grow in a monolayer with an epithelial morphology and exhibit positive staining for cytokeratin. EPYP-1 cells exhibited positive staining with anti-prostatic acid phosphatase antibody and expressed Integrin α6,B1 on their plasma membrane. They did nor demonstrate Dil-Ac-LDL uptake as an endothelial marker. Both testosterone and dihydrotestosterone stimulated the growth of EPYP-1. Genetically EPYP-1 was aneuploid, however no tumors formed after subcutaneous and intra-prostatic injection of the cells in Copenhagen rats. This immortalized cell line of Copenhagen rat prostate epithelial origin may be suitable for studying early events in the conversion of cells to tumorigenicity.
KW - Copenhagen rat
KW - Immortalization
KW - Prostate cancer
KW - Prostate epithelium
KW - T-antigen
UR - http://www.scopus.com/inward/record.url?scp=0029551978&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029551978&partnerID=8YFLogxK
M3 - Article
C2 - 8900919
AN - SCOPUS:0029551978
VL - 9
SP - 427
EP - 431
JO - In Vivo
JF - In Vivo
SN - 0258-851X
IS - 5
ER -