Establishment of an immortalized Copenhagen rat prostate epithelial cell line

K. Yamazaki, K. J. Pienta

Research output: Contribution to journalArticle

Abstract

An immortal cell line of Copenhagen rat prostate epithelium was established after transfection of primary cultured cells with SV40 DNA and designated EPYP-1. The established cells have continued to proliferate more than 70 population doublings, have not undergone senescence, and stain positively for SV40 T-antigen in their nuclei by immunohistochemistry. They grow in a monolayer with an epithelial morphology and exhibit positive staining for cytokeratin. EPYP-1 cells exhibited positive staining with anti-prostatic acid phosphatase antibody and expressed Integrin α6,B1 on their plasma membrane. They did nor demonstrate Dil-Ac-LDL uptake as an endothelial marker. Both testosterone and dihydrotestosterone stimulated the growth of EPYP-1. Genetically EPYP-1 was aneuploid, however no tumors formed after subcutaneous and intra-prostatic injection of the cells in Copenhagen rats. This immortalized cell line of Copenhagen rat prostate epithelial origin may be suitable for studying early events in the conversion of cells to tumorigenicity.

Original languageEnglish (US)
Pages (from-to)427-431
Number of pages5
JournalIn Vivo
Volume9
Issue number5
StatePublished - Dec 1 1995
Externally publishedYes

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Keywords

  • Copenhagen rat
  • Immortalization
  • Prostate cancer
  • Prostate epithelium
  • T-antigen

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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